Evidence for the presence of clofazimine and its distribution in the healthy mouse brain.

Authors :: Baijnath S
Naiker S
Shobo A
Moodley C
Adamson J
Ngcobo B
Bester LA
Singh S
Kruger HG
Naicker T
Govender T
Source :: Journal of molecular histology [J Mol Histol] 2015 Oct; Vol. 46 (4-5), pp. 439-42. Date of Electronic Publication: 2015 Jul 25.
Publication Year :: 2015
Abstract: This is the first report of clofazimine (CFZ) penetration and distribution in normal mouse brain. Mice were administered 25 mg/kg CFZ or 100 mg/kg CFZ orally, daily for 2 weeks. Animals were sacrificed and blood and brain tissues were harvested. Liquid chromatography tandem mass spectrometry showed high concentrations of CFZ in homogenized brain, with 100 mg/kg dose having significantly higher concentration than 25 mg/kg. Matrix-assisted laser desorption/ionization spectrometric imaging of brain sections showed widespread tissue distribution of CFZ. Our results show dose dependent localization in brain.

Subjects :: Animals
Anti-Inflammatory Agents administration & dosage
Clofazimine administration & dosage
Mice
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Time Factors
Tissue Distribution
Anti-Inflammatory Agents pharmacokinetics
Brain drug effects
Brain metabolism
Clofazimine pharmacokinetics

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