Back to Search
Start Over
Temperature-sensitive mutants of vaccinia virus. I. Isolation and preliminary characterization.
Temperature-sensitive mutants of vaccinia virus. I. Isolation and preliminary characterization.
- Source :
-
Acta virologica [Acta Virol] 1978 Mar; Vol. 22 (2), pp. 81-90. - Publication Year :
- 1978
-
Abstract
- Fifty-seven temperature-sensitive (ts) mutants of a neurotropic variant of vaccinia virus were obtained. A comparison of the effectiveness of 6 different mutagens showed that in in vivo experiments nitrosomethylurea induced 10%, nitrosoguanidine 8%, and bromodeoxyuridine 2.5% ts mutants. In vitro, at the same degree of inactivation, UV irradiation, hydroxylamine and nitrous acid induced 2.5% ts mutants. Out of 49 mutants studied, 27 had a DNA+ phenotype, 19 a DNA+/-and 3 a DNA- phenotype. Under permissive conditions, two thirds of all the mutants tested formed thermolabile virions. In experiments on genetic recombination, the recombination frequency between different pairs was from 2.8 to 57%. Seven of the mutants were plotted on the virus genetic map in a certain sequence.
Details
- Language :
- English
- ISSN :
- 0001-723X
- Volume :
- 22
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Acta virologica
- Publication Type :
- Academic Journal
- Accession number :
- 26208