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Universal Fluorescence Biosensor Platform Based on Graphene Quantum Dots and Pyrene-Functionalized Molecular Beacons for Detection of MicroRNAs.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2015 Aug 05; Vol. 7 (30), pp. 16152-6. Date of Electronic Publication: 2015 Jul 27. - Publication Year :
- 2015
-
Abstract
- A novel biosensor platform was developed for detection of microRNAs (miRNAs) based on graphene quantum dots (GQDs) and pyrene-functionalized molecular beacon probes (py-MBs). Pyrene was introduced to trigger specifically fluorescence resonance energy transfer (FRET) between GQDs and fluorescent dyes labeled on py-MBs, and the unique fluorescent intensity change produced a novel signal for detection of the target. The platform realized detection of miRNAs in a wide range from 0.1 nM to 200 nM with great discrimination abilities, as well as multidetection of different kinds of miRNAs, which paved a brand new way for miRNA detection based on GQDs.
- Subjects :
- Equipment Design
Equipment Failure Analysis
Graphite chemistry
Molecular Probe Techniques instrumentation
Molecular Probes chemistry
Reproducibility of Results
Sensitivity and Specificity
Biosensing Techniques instrumentation
Fluorescence Resonance Energy Transfer instrumentation
MicroRNAs analysis
MicroRNAs genetics
Pyrenes chemistry
Quantum Dots
Subjects
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 7
- Issue :
- 30
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 26200323
- Full Text :
- https://doi.org/10.1021/acsami.5b04773