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Piezo Is Essential for Amiloride-Sensitive Stretch-Activated Mechanotransduction in Larval Drosophila Dorsal Bipolar Dendritic Sensory Neurons.
- Source :
-
PloS one [PLoS One] 2015 Jul 17; Vol. 10 (7), pp. e0130969. Date of Electronic Publication: 2015 Jul 17 (Print Publication: 2015). - Publication Year :
- 2015
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Abstract
- Stretch-activated afferent neurons, such as those of mammalian muscle spindles, are essential for proprioception and motor co-ordination, but the underlying mechanisms of mechanotransduction are poorly understood. The dorsal bipolar dendritic (dbd) sensory neurons are putative stretch receptors in the Drosophila larval body wall. We have developed an in vivo protocol to obtain receptor potential recordings from intact dbd neurons in response to stretch. Receptor potential changes in dbd neurons in response to stretch showed a complex, dynamic profile with similar characteristics to those previously observed for mammalian muscle spindles. These profiles were reproduced by a general in silico model of stretch-activated neurons. This in silico model predicts an essential role for a mechanosensory cation channel (MSC) in all aspects of receptor potential generation. Using pharmacological and genetic techniques, we identified the mechanosensory channel, DmPiezo, in this functional role in dbd neurons, with TRPA1 playing a subsidiary role. We also show that rat muscle spindles exhibit a ruthenium red-sensitive current, but found no expression evidence to suggest that this corresponds to Piezo activity. In summary, we show that the dbd neuron is a stretch receptor and demonstrate that this neuron is a tractable model for investigating mechanisms of mechanotransduction.
- Subjects :
- Amiloride pharmacology
Animals
Computer Simulation
Dendrites drug effects
Dendrites metabolism
Dendrites ultrastructure
Drosophila Proteins metabolism
Drosophila melanogaster drug effects
Evoked Potentials, Somatosensory physiology
Gene Expression Regulation
Ion Channels metabolism
Larva drug effects
Larva physiology
Mechanoreceptors drug effects
Mechanoreceptors ultrastructure
Models, Biological
Muscle Spindles physiology
Proprioception physiology
Rats
Sensory Receptor Cells drug effects
Sensory Receptor Cells ultrastructure
Stress, Mechanical
TRPA1 Cation Channel
TRPC Cation Channels metabolism
Drosophila Proteins genetics
Drosophila melanogaster physiology
Ion Channels genetics
Mechanoreceptors metabolism
Mechanotransduction, Cellular
Sensory Receptor Cells metabolism
TRPC Cation Channels genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 26186008
- Full Text :
- https://doi.org/10.1371/journal.pone.0130969