Back to Search
Start Over
Effective transfer of a 47 kb NDM-1-positive plasmid among Acinetobacter species.
- Source :
-
The Journal of antimicrobial chemotherapy [J Antimicrob Chemother] 2015 Oct; Vol. 70 (10), pp. 2734-8. Date of Electronic Publication: 2015 Jul 14. - Publication Year :
- 2015
-
Abstract
- Objectives: To investigate the link between two NDM-1-positive Acinetobacter isolates from the same hospital, the plasmid profiles of the isolates were examined. These two isolates were found from a surveillance programme within 3 months from two patients without obvious physical contact or hospitalization time overlap.<br />Methods: Antimicrobial susceptibility tests, genome sequencing of both isolates and plasmid transfer experiments were performed. A comparative study of similar plasmids was performed using BLAST analysis.<br />Results: The antimicrobial susceptibility of the isolates (Acinetobacter soli M131 and Acinetobacter pittii MS32) and their Escherichia coli transconjugants revealed a conjugative plasmid that carried the carbapenem resistance determinant. Eleven plasmids were observed in M131 and three in MS32. Each isolate shared an identical plasmid that carried the blaNDM-1 gene. This 47 271 bp plasmid harbours a conserved blaNDM-1-containing region that is flanked by ISAba125 and ISAba11 elements, and also contains a Ti-type conjugative operon. The plasmid is nearly identical in sequence to those of Acinetobacter isolates from China. In contrast to the mobilization of the blaNDM-1 sequence in Enterobacteriaceae, which is mainly by transposition, this plasmid moves as a whole among Acinetobacter species. Consistently, this plasmid was found to transfer effectively by in vitro conjugation to several Acinetobacter species.<br />Conclusions: The clinical and laboratory findings suggest that Acinetobacter species may serve as a reservoir of this blaNDM-1 plasmid. Our study demonstrates the potential of applying genome sequencing to the surveillance of antimicrobial-resistant bacteria.<br /> (© The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.)
- Subjects :
- Acinetobacter drug effects
Anti-Bacterial Agents pharmacology
Cross Infection
DNA Transposable Elements
Gene Order
Genome, Bacterial
Humans
Microbial Sensitivity Tests
beta-Lactam Resistance
Acinetobacter genetics
Acinetobacter Infections microbiology
Conjugation, Genetic
Gene Transfer, Horizontal
Plasmids genetics
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2091
- Volume :
- 70
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- The Journal of antimicrobial chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 26180132
- Full Text :
- https://doi.org/10.1093/jac/dkv191