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PLK1 regulates spindle formation kinetics and APC/C activation in mouse zygote.
- Source :
-
Zygote (Cambridge, England) [Zygote] 2016 Jun; Vol. 24 (3), pp. 338-45. Date of Electronic Publication: 2015 Jul 15. - Publication Year :
- 2016
-
Abstract
- Polo-like kinase 1 (PLK1) is involved in essential events of cell cycle including mitosis in which it participates in centrosomal microtubule nucleation, spindle bipolarity establishment and cytokinesis. Although PLK1 function has been studied in cycling cancer cells, only limited data are known about its role in the first mitosis of mammalian zygotes. During the 1-cell stage of mouse embryo development, the acentriolar spindle is formed and the shift from acentriolar to centrosomal spindle formation progresses gradually throughout the preimplantation stage, thus providing a unique possibility to study acentriolar spindle formation. We have shown previously that PLK1 activity is not essential for entry into first mitosis, but is required for correct spindle formation and anaphase onset in 1-cell mouse embryos. In the present study, we extend this knowledge by employing quantitative confocal live cell imaging to determine spindle formation kinetics in the absence of PLK1 activity and answer the question whether metaphase arrest at PLK1-inhibited embryos is associated with low anaphase-promoting complex/cyclosome (APC/C) activity and consequently high securin level. We have shown that inhibition of PLK1 activity induces a delay in onset of acentriolar spindle formation during first mitosis. Although these PLK1-inhibited 1-cell embryos were finally able to form a bipolar spindle, not all chromosomes were aligned at the metaphase equator. PLK1-inhibited embryos were arrested in metaphase without any sign of APC/C activation with high securin levels. Our results document that PLK1 controls the onset of spindle assembly and spindle formation, and is essential for APC/C activation before anaphase onset in mouse zygotes.
- Subjects :
- Anaphase
Animals
Blastocyst
Cell Cycle Proteins antagonists & inhibitors
Centrosome metabolism
Female
Kinetics
Kinetochores metabolism
Male
Mice
Microscopy, Confocal
Mitosis
Protein Serine-Threonine Kinases antagonists & inhibitors
Proto-Oncogene Proteins antagonists & inhibitors
Pteridines pharmacology
Time-Lapse Imaging
Zygote drug effects
Polo-Like Kinase 1
Anaphase-Promoting Complex-Cyclosome metabolism
Cell Cycle Proteins metabolism
Protein Serine-Threonine Kinases metabolism
Proto-Oncogene Proteins metabolism
Spindle Apparatus metabolism
Zygote metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8730
- Volume :
- 24
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Zygote (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 26174739
- Full Text :
- https://doi.org/10.1017/S0967199415000246