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Oil-in-Water Emulsion MF59 Increases Germinal Center B Cell Differentiation and Persistence in Response to Vaccination.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2015 Aug 15; Vol. 195 (4), pp. 1617-27. Date of Electronic Publication: 2015 Jul 13. - Publication Year :
- 2015
-
Abstract
- Induction of persistent protective immune responses is a key attribute of a successful vaccine formulation. MF59 adjuvant, an oil-in-water emulsion used in human vaccines, is known to induce persistent high-affinity functional Ab titers and memory B cells, but how it really shapes the Ag-specific B cell compartment is poorly documented. In this study, we characterized the Ab- and Ag-specific B cell compartment in wild-type mice immunized with HlaH35L, a Staphylococcus aureus Ag known to induce measurable functional Ab responses, formulated with MF59 or aluminum salts, focusing on germinal centers (GC) in secondary lymphoid organs. Taking advantage of single-cell flow cytometry analyses, HlaH35L-specific B cells were characterized for the expression of CD38 and GL-7, markers of memory and GC, respectively, and for CD80 and CD73 activation markers. We demonstrated that immunization with MF59-, but not aluminum salt-adjuvanted HlaH35L, induced expanded Ag-specific CD73(+)CD80(-) GC B cells in proximal- and distal-draining lymph nodes, and promoted the persistence of GC B cells, detected up to 4 mo after immunization. In addition to increasing GC B cells, MF59-adjuvanted HlaH35L also increased the frequency of T follicular helper cells. This work extends previous knowledge regarding adaptive immune responses to MF59-adjuvanted vaccines, and, to our knowledge, for the first time an adjuvant used in human licensed products is shown to promote strong and persistent Ag-specific GC responses that might benefit the rational design of new vaccination strategies.<br /> (Copyright © 2015 by The American Association of Immunologists, Inc.)
- Subjects :
- Adjuvants, Immunologic
Animals
Antibodies, Bacterial immunology
Antibody Specificity immunology
Antigens, CD metabolism
B-Lymphocytes metabolism
Bacterial Toxins immunology
Chemotaxis, Leukocyte immunology
Female
Hemolysin Proteins immunology
Immunophenotyping
Lymph Nodes immunology
Lymphocyte Activation immunology
Mice
Phenotype
Staphylococcal Vaccines
B-Lymphocytes cytology
B-Lymphocytes immunology
Cell Differentiation
Germinal Center cytology
Germinal Center immunology
Polysorbates
Squalene immunology
Vaccination
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 195
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 26170383
- Full Text :
- https://doi.org/10.4049/jimmunol.1402604