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Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity.
- Source :
-
Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2015 Oct 15; Vol. 167 (3-4), pp. 171-7. Date of Electronic Publication: 2015 Jun 27. - Publication Year :
- 2015
-
Abstract
- Insect bite hypersensitivity (IBH) is a seasonal recurrent skin allergy of horses caused by IgE-mediated reactions to allergens present in the saliva of biting insects of the genus Culicoides, and possibly also Simulium and Stomoxys species. In this work we show that protein microarrays containing complex extracts and pure proteins, including recombinant Culicoides allergens, can be used as a powerful technique for the diagnosis of IBH. Besides the obvious advantages such as general profiling and use of few microliters of samples, this microarray technique permits automation and allows the generation of mathematical models with the calculation of individual risk profiles that can support the clinical diagnosis of allergic diseases. After selection of variables on influence on the projection (VIP), the observed values of sensitivity and specificity were 1.0 and 0.967, respectively. This confirms the highly discriminatory power of this approach for IBH and made it possible to attain a robust predictive mathematical model for this disease. It also further demonstrates the specificity of the protein array method on identifying a particular IgE-mediated disease when the sensitising allergen group is known.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Allergens
Animals
Case-Control Studies
Ceratopogonidae immunology
Diagnosis, Computer-Assisted veterinary
Female
Horses
Hypersensitivity diagnosis
Hypersensitivity immunology
Immunoglobulin E blood
Insect Bites and Stings diagnosis
Insect Bites and Stings immunology
Male
Mathematical Concepts
Models, Immunological
Protein Array Analysis
Skin immunology
Horse Diseases diagnosis
Horse Diseases immunology
Hypersensitivity veterinary
Insect Bites and Stings veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2534
- Volume :
- 167
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Veterinary immunology and immunopathology
- Publication Type :
- Academic Journal
- Accession number :
- 26163936
- Full Text :
- https://doi.org/10.1016/j.vetimm.2015.06.013