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Evaluation of GenoType MTBDRplus for the rapid detection of drug-resistant tuberculosis in Ghana.
- Source :
-
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease [Int J Tuberc Lung Dis] 2015 Aug; Vol. 19 (8), pp. 954-959. - Publication Year :
- 2015
-
Abstract
- Background: Rapid but simple diagnostic tools for the detection of drug-resistant (DR) tuberculosis (TB) have been acknowledged as being important for its effective management and control.<br />Objective: To establish a molecular line-probe assay (GenoType MTBDRplus) for detecting DR-TB in Ghana.<br />Method: We first screened 113 Mycobacterium tuberculosis isolates using the indirect proportion method and MTBDRplus. The rpoB and katG genes and the promoter regions of oxyR-ahpC and inhA were sequenced to identify mutations in isolates found to be resistant on phenotypic drug susceptibility testing and/or MTBDRplus. We then analysed an additional 412 isolates using only MTBDRplus.<br />Results: Respectively 43 (8.2%) and 8 (1.5%) isolates were resistant to isoniazid (INH) and rifampicin (RMP), while 8 (1.5%) were multidrug-resistant. In resistant isolates, mutations in codon 450 of rpoB and codon 315 of katG, conferring resistance to respectively RMP and INH, dominated. We found two RMP-resistant isolates with a S450L substitution, each harbouring an additional mutation at S388L and Q409R. Using phenotypic testing as gold standard, the MTBDRplus assay showed a sensitivity and specificity in the detection of RMP and INH resistance and multidrug resistance of respectively 100% and 100%, 83.3% and 100%, and 100% and 100%.<br />Conclusion: The high sensitivity of MTBDRplus makes it a valuable addition to the conventional TB diagnostic algorithm in Ghana.
- Subjects :
- Cross-Sectional Studies
Drug Resistance, Multiple, Bacterial
Ghana
Humans
Isoniazid pharmacology
Microbial Sensitivity Tests
Molecular Diagnostic Techniques methods
Mutation
Mycobacterium tuberculosis genetics
Phenotype
Rifampin pharmacology
Sensitivity and Specificity
Tuberculosis, Multidrug-Resistant microbiology
Tuberculosis, Pulmonary microbiology
Antitubercular Agents pharmacology
Mycobacterium tuberculosis isolation & purification
Tuberculosis, Multidrug-Resistant diagnosis
Tuberculosis, Pulmonary diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1815-7920
- Volume :
- 19
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease
- Publication Type :
- Academic Journal
- Accession number :
- 26162362
- Full Text :
- https://doi.org/10.5588/ijtld.14.0864