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Optimization of high solids fed-batch saccharification of sugarcane bagasse based on system viscosity changes.
- Source :
-
Journal of biotechnology [J Biotechnol] 2015 Oct 10; Vol. 211, pp. 5-9. Date of Electronic Publication: 2015 Jul 03. - Publication Year :
- 2015
-
Abstract
- Viscosity trends in alkali-pretreated sugarcane bagasse (SCB) slurries undergoing high solids fed-batch enzymatic hydrolysis were measured for a range of solids loading from 15% to 36%. Solids liquefaction times were related to system viscosity changes. The viscosity decreased quickly for low solids loading, and increased with increasing solids content. Fed-batch hydrolysis was initiated with 15% solids loading, and an additional 8%, 7% and 6% were successively added after the system viscosity decreased to stable values to achieve a final solids content of 36%. Two enzyme-adding modes with 8.5FPU/g solid were investigated. The batch mode with all enzyme being added at the beginning of the reaction produced the highest yields, with approximately 231.7g/L total sugars and 134.9g/L glucose being obtained after 96h with nearly 60% of the final glucan conversion rate. This finding indicates that under the right conditions, the fed-batch strategy might be a plausible way to produce high sugars under high solids.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-4863
- Volume :
- 211
- Database :
- MEDLINE
- Journal :
- Journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 26150018
- Full Text :
- https://doi.org/10.1016/j.jbiotec.2015.06.422