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Disruption of the Arabidopsis Defense Regulator Genes SAG101, EDS1, and PAD4 Confers Enhanced Freezing Tolerance.
- Source :
-
Molecular plant [Mol Plant] 2015 Oct 05; Vol. 8 (10), pp. 1536-49. Date of Electronic Publication: 2015 Jul 03. - Publication Year :
- 2015
-
Abstract
- In Arabidopsis, three lipase-like regulators, SAG101, EDS1, and PAD4, act downstream of resistance protein-associated defense signaling. Although the roles of SAG101, EDS1, and PAD4 in biotic stress have been extensively studied, little is known about their functions in plant responses to abiotic stresses. Here, we show that SAG101, EDS1, and PAD4 are involved in the regulation of freezing tolerance in Arabidopsis. With or without cold acclimation, the sag101, eds1, and pad4 single mutants, as well as their double mutants, exhibited similarly enhanced tolerance to freezing temperatures. Upon cold exposure, the sag101, eds1, and pad4 mutants showed increased transcript levels of C-REPEAT/DRE BINDING FACTORs and their regulons compared with the wild type. Moreover, freezing-induced cell death and accumulation of hydrogen peroxide were ameliorated in sag101, eds1, and pad4 mutants. The sag101, eds1, and pad4 mutants had much lower salicylic acid (SA) and diacylglycerol (DAG) contents than the wild type, and exogenous application of SA and DAG compromised the freezing tolerance of the mutants. Furthermore, SA suppressed the cold-induced expression of DGATs and DGKs in the wild-type leaves. These findings indicate that SAG101, EDS1, and PAD4 are involved in the freezing response in Arabidopsis, at least in part, by modulating the homeostasis of SA and DAG.<br /> (Copyright © 2015 The Author. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Arabidopsis genetics
Arabidopsis Proteins genetics
Carboxylic Ester Hydrolases genetics
DNA-Binding Proteins genetics
Diglycerides metabolism
Gene Expression Regulation, Plant genetics
Gene Expression Regulation, Plant physiology
Plant Diseases
Salicylic Acid metabolism
Arabidopsis metabolism
Arabidopsis physiology
Arabidopsis Proteins metabolism
Carboxylic Ester Hydrolases metabolism
DNA-Binding Proteins metabolism
Freezing
Subjects
Details
- Language :
- English
- ISSN :
- 1752-9867
- Volume :
- 8
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Molecular plant
- Publication Type :
- Academic Journal
- Accession number :
- 26149542
- Full Text :
- https://doi.org/10.1016/j.molp.2015.06.009