Back to Search Start Over

Ragged-red fibres detected in paraffin sections by a monoclonal antibody to inner mitochondrial membrane.

Ragged-red fibres detected in paraffin sections by a monoclonal antibody to inner mitochondrial membrane.

Authors :
Paulus W
Peiffer J
Roggendorf W
Wiedmann KH
Schröder JM
Source :
Journal of neurology [J Neurol] 1989 Dec; Vol. 236 (8), pp. 482-3.
Publication Year :
1989

Abstract

An immunohistochemical method is reported using the M-II68 monoclonal antibody, which detects mitochondrial accumulations ("ragged-red fibres") in routinely processed (formalin-fixed, paraffin-embedded) muscle tissue. Ten cases with electron-microscopically and histochemically proven mitochondrial myopathy featured 4% to 24% ragged-red fibres. In a series of 50 muscle biopsies without mitochondrial myopathy, scattered ragged-red fibres (less than 0.1%) were present in a few normal and pathological muscles. The immunohistochemical method is specific for mitochondria, does not require frozen tissue and permits rapid examination of large areas.

Details

Language :
English
ISSN :
0340-5354
Volume :
236
Issue :
8
Database :
MEDLINE
Journal :
Journal of neurology
Publication Type :
Academic Journal
Accession number :
2614495
Full Text :
https://doi.org/10.1007/BF00328512