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The role of matrix metalloproteinases and cysteine-cathepsins on the progression of dentine erosion.
- Source :
-
Archives of oral biology [Arch Oral Biol] 2015 Sep; Vol. 60 (9), pp. 1340-5. Date of Electronic Publication: 2015 Jun 22. - Publication Year :
- 2015
-
Abstract
- Objective: To evaluate in vitro the effect of the inhibition of endogenous dentinal enzymes (matrix metalloproteinases-MMPs and cysteine cathepsins-CCs) on dentine erosion.<br />Design: Dentine blocks (4mm×4mm×2mm) from sound human teeth were randomly divided into 7 groups (n=17) according to the treatment: MMP- and CC-inhibitor chlorhexidine digluconate (CHX, 10mM); MMP-inhibitor galardin (G, 0.2mM); specific cathepsin B inhibitor (CCB, 0.2mM); non-specific CC inhibitor (CCE-64, 0.5μM); fluoride (F, 1.23% NaF); placebo (P) and untreated (UT). Inhibitors were applied as gels once for 1min. Specimens were submitted to 5 days of pH cycling including the erosive challenge (Coke, pH 2.64, 90s/day) and remineralisation (artificial saliva). Demineralised organic surface loss was determined profilometrically. Demineralised organic matrix (DOM) was removed with collagenase and the profile was re-evaluated in the absence of collagen fibrils. The differences in profilometric results and DOM thickness among the groups were analysed with ANOVA and Tukey's test (p<0.05).<br />Results: Loss of demineralised tissue (μm, mean±SD) was: CHX 8.4±1.7 b, G 8.6±1.9 b, CCB 9.6±1.4 a, CCE-64 9.9±1.3 a, F 9.9±1.7 a, P 10.9±2.2 a, UT 11.0±1.5 a. Loss of mineralised tissue was: CHX 15.4±2.2 b, G 16.0±1.8 b, CCB 17.6±2.4 a, CCE-64 17.6±2.0 a, F 17.3±2.8 a, P 19.1±2.1 a, UT 18.9±2.4 a. MMP-inhibitors significantly reduced organic matrix and mineral loss in comparison to all the other groups (p<0.05). No statistically significant differences were found in the thickness of the remaining DOM (p=0.845).<br />Conclusion: Dentine endogenous MMPs seem to be the main enzymes responsible for DOM loss and erosion.<br /> (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Subjects :
- Chlorhexidine pharmacology
Dentin drug effects
Disease Progression
Humans
In Vitro Techniques
Molar, Third
Random Allocation
Cathepsins antagonists & inhibitors
Chlorhexidine analogs & derivatives
Cysteine antagonists & inhibitors
Dipeptides pharmacology
Matrix Metalloproteinase Inhibitors pharmacology
Matrix Metalloproteinases physiology
Sodium Fluoride pharmacology
Tooth Erosion prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1506
- Volume :
- 60
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Archives of oral biology
- Publication Type :
- Academic Journal
- Accession number :
- 26134516
- Full Text :
- https://doi.org/10.1016/j.archoralbio.2015.06.011