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Functional and structural responses of soil N-cycling microbial communities to the herbicide mesotrione: a dose-effect microcosm approach.
- Source :
-
Environmental science and pollution research international [Environ Sci Pollut Res Int] 2016 Mar; Vol. 23 (5), pp. 4207-17. Date of Electronic Publication: 2015 Jun 28. - Publication Year :
- 2016
-
Abstract
- Microbial communities driving the nitrogen cycle contribute to ecosystem services such as crop production and air, soil, and water quality. The responses to herbicide stress of ammonia-oxidizing and ammonia-denitrifying microbial communities were investigated by an analysis of changes in structure-function relationships. Their potential activities, abundances (quantitative PCR), and genetic structure (denaturing gradient gel electrophoresis) were assessed in a microcosm experiment. The application rate (1 × FR, 0.45 μg g(-1) soil) of the mesotrione herbicide did not strongly affect soil N-nutrient dynamics or microbial community structure and abundances. Doses of the commercial product Callisto® (10 × FR and 100 × FR) or pure mesotrione (100 × FR) exceeding field rates induced short-term inhibition of nitrification and a lasting stimulation of denitrification. These effects could play a part in the increase in soil ammonium content and decrease in nitrate contents observed in treated soils. These functional impacts were mainly correlated with abundance shifts of ammonia-oxidizing Bacteria (AOB) and Archaea (AOA) or denitrifying bacteria. The sustained restoration of nitrification activity, from day 42 in the 100 × FR-treated soils, was likely promoted by changes in the community size and composition of AOB, which suggests a leading role, rather than AOA, for soil nitrification restoration after herbicide stress. This ecotoxicological community approach provides a nonesuch multiparameter assessment of responses of N-cycling microbial guilds to pesticide stress.
- Subjects :
- Ammonia
Archaea drug effects
Archaea genetics
Betaproteobacteria drug effects
Betaproteobacteria genetics
Denaturing Gradient Gel Electrophoresis
Dose-Response Relationship, Drug
Ecosystem
Microbial Consortia genetics
Nitrification
Oxidation-Reduction
Cyclohexanones toxicity
Herbicides toxicity
Microbial Consortia drug effects
Nitrogen Cycle drug effects
Soil chemistry
Soil Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1614-7499
- Volume :
- 23
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Environmental science and pollution research international
- Publication Type :
- Academic Journal
- Accession number :
- 26122568
- Full Text :
- https://doi.org/10.1007/s11356-015-4797-8