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A Plasmodium α/β-hydrolase modulates the development of invasive stages.
- Source :
-
Cellular microbiology [Cell Microbiol] 2015 Dec; Vol. 17 (12), pp. 1848-67. Date of Electronic Publication: 2015 Jul 24. - Publication Year :
- 2015
-
Abstract
- The bud emergence (BEM)46 proteins are evolutionarily conserved members of the α/β-hydrolase superfamily, which includes enzymes with diverse functions and a wide range of substrates. Here, we identified a Plasmodium BEM46-like protein (PBLP) and characterized it throughout the life cycle of the rodent malaria parasite Plasmodium yoelii. The Plasmodium BEM46-like protein is shown to be closely associated with the parasite plasma membrane of asexual erythrocytic stage schizonts and exo-erythrocytic schizonts; however, PBLP localizes to unique intracellular structures in sporozoites. Generation and analysis of P. yoelii knockout (Δpblp) parasite lines showed that PBLP has an important role in erythrocytic stage merozoite development with Δpblp parasites forming fewer merozoites during schizogony, which results in decreased parasitemia when compared with wild-type (WT) parasites. Δpblp parasites showed no defects in gametogenesis or transmission to mosquitoes; however, because they formed fewer oocysts there was a reduction in the number of developed sporozoites in infected mosquitoes when compared with WT. Although Δpblp sporozoites showed no apparent defect in mosquito salivary gland infection, they showed decreased infectivity in hepatocytes in vitro. Similarly, mice infected with Δpblp sporozoites exhibited a delay in the onset of blood-stage patency, which is likely caused by reduced sporozoite infectivity and a discernible delay in exo-erythrocytic merozoite formation. These data are consistent with the model that PBLP has an important role in parasite invasive-stage morphogenesis throughout the parasite life cycle.<br /> (© 2015 John Wiley & Sons Ltd.)
- Subjects :
- Amino Acid Sequence
Animals
Cell Line
Cell Membrane enzymology
Culicidae
Gene Deletion
Hydrolases genetics
Membrane Proteins genetics
Membrane Proteins metabolism
Merozoites enzymology
Merozoites growth & development
Mice
Mice, Inbred BALB C
Models, Molecular
Molecular Sequence Data
Plasmodium yoelii genetics
Plasmodium yoelii growth & development
Sporozoites enzymology
Sporozoites growth & development
Hydrolases metabolism
Plasmodium yoelii enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1462-5822
- Volume :
- 17
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Cellular microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 26118838
- Full Text :
- https://doi.org/10.1111/cmi.12477