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In Vitro infection of human dura-mater fibroblasts with Staphylococcus aureus: colonization and reactive production of IL-1beta.
- Source :
-
Neurological research [Neurol Res] 2015 Oct; Vol. 37 (10), pp. 867-73. Date of Electronic Publication: 2015 Jun 24. - Publication Year :
- 2015
-
Abstract
- Objective: Post-operative meningitis, caused mainly by Staphylococcus aureus and Gram-negative rods, is a life-threatening complication after neurosurgery, and its pathogenesis is far from clear. The purpose of this work was to study the experimental infection of human dura-mater fibroblasts and whole human dura by S. aureus.<br />Methods: In vitro cultures of human dura-mater fibroblasts and organotypic cultures of small pieces of human dura mater were inoculated with a human-derived S. aureus strain. The pattern of bacterial infection as well as cytokines secretion by the infected fibroblasts was studied.<br />Results: Our results suggest that colonisation of human dura-mater fibroblasts in culture and whole dura-mater tissue by S. aureus includes bacterial growth on the cell surface, fibroblast intracellular invasion by bacteria and a significant synthesis of interleukin 1beta (IL-1beta) by the infected cells.<br />Conclusion: This is the first report of human dura-mater fibroblast infection by S. aureus. Hopefully, these results can lead to a better understanding of the pathogenesis of meningitis caused by this bacterial species and to a more rational therapeutic approach.
- Subjects :
- Cells, Cultured
Dura Mater metabolism
Dura Mater ultrastructure
Fibroblasts metabolism
Fibroblasts ultrastructure
Humans
In Vitro Techniques
Interleukin-1beta metabolism
Organ Culture Techniques
Staphylococcus aureus ultrastructure
Dura Mater microbiology
Fibroblasts microbiology
Staphylococcal Infections metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1743-1328
- Volume :
- 37
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Neurological research
- Publication Type :
- Academic Journal
- Accession number :
- 26104574
- Full Text :
- https://doi.org/10.1179/1743132815Y.0000000065