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3,4-dihydroxyphenylethanol suppresses irradiation-induced pulmonary fibrosis in adult rats.
- Source :
-
International journal of clinical and experimental pathology [Int J Clin Exp Pathol] 2015 Apr 01; Vol. 8 (4), pp. 3441-50. Date of Electronic Publication: 2015 Apr 01 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- The present study demonstrates the effect of DHPEA on suppression of irradiation-induced pulmonary fibrosis. A (60)Co irradiator was used to induce pulmonary fibrosis in a rat model at a dose of 22 Gy. The rats of the treatment and positive control group were intraperitoneally injected DHPEA (10 mg/kg) or dexamethasone (DEX; 5 mg/kg) daily for 30 days. Hydroxyproline assay was used to evaluate the fibrosis of pulmonary and lung tissue sections after irradiation. Hematoxylin and eosin (H&E) and Masson's trichrome stained lung section were used for alveolitis and fibrosis score analyses, respectively. Immunohistochemistry was used for surfactant protein-B (SPB) and α-SMA expression analysis. Western blot analysis was employed for analysis of nuclear transcription factor NF-E2-related factor 2 (Nrf-2) and its associated antioxidant enzymes like heme oxygenase-1 (HO-1) and NAD (P) H: quinone oxidoreductase-1 (NQO-1). Results revealed a significant decrease in mortality rates and lung index scores, decreased collagen deposition, reduced MDA content and enhanced superoxide dismutase (SOD) activity in DHPEA treated rats compared to DEX-treated rats. DHPEA treatment also inhibited (myo) fibroblast proliferation, and regulated serum levels of TGF-β1, IL-6, IL-10, and TNF-α. In addition, DHPEA-treatment activated Nrf-2 and its downstream antioxidant enzymes HO-1 and NQO-1. Thus DHPEA can be a promising agent for the suppression of irradiation-induced pulmonary fibrosis.
- Subjects :
- Animals
Anti-Inflammatory Agents metabolism
Antioxidants chemistry
Antioxidants pharmacology
Cytokines blood
Dexamethasone pharmacology
Disease Models, Animal
Heme Oxygenase-1 drug effects
Heme Oxygenase-1 metabolism
Humans
Lung drug effects
Lung pathology
Male
NAD(P)H Dehydrogenase (Quinone) drug effects
NAD(P)H Dehydrogenase (Quinone) metabolism
NF-E2-Related Factor 2 drug effects
NF-E2-Related Factor 2 metabolism
Phenylethyl Alcohol chemistry
Phenylethyl Alcohol pharmacology
Phenylethyl Alcohol therapeutic use
Pulmonary Fibrosis drug therapy
Pulmonary Fibrosis metabolism
Pulmonary Fibrosis pathology
Radiation Injuries, Experimental drug therapy
Radiation Injuries, Experimental metabolism
Radiation Injuries, Experimental pathology
Rats
Rats, Sprague-Dawley
Antioxidants therapeutic use
Phenylethyl Alcohol analogs & derivatives
Pulmonary Fibrosis prevention & control
Radiation Injuries, Experimental prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1936-2625
- Volume :
- 8
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- International journal of clinical and experimental pathology
- Publication Type :
- Academic Journal
- Accession number :
- 26097528