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End-ischemic machine perfusion reduces bile duct injury in donation after circulatory death rat donor livers independent of the machine perfusion temperature.
- Source :
-
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society [Liver Transpl] 2015 Oct; Vol. 21 (10), pp. 1300-11. - Publication Year :
- 2015
-
Abstract
- A short period of oxygenated machine perfusion (MP) after static cold storage (SCS) may reduce biliary injury in donation after cardiac death (DCD) donor livers. However, the ideal perfusion temperature for protection of the bile ducts is unknown. In this study, the optimal perfusion temperature for protection of the bile ducts was assessed. DCD rat livers were preserved by SCS for 6 hours. Thereafter, 1 hour of oxygenated MP was performed using either hypothermic machine perfusion, subnormothermic machine perfusion, or with controlled oxygenated rewarming (COR) conditions. Subsequently, graft and bile duct viability were assessed during 2 hours of normothermic ex situ reperfusion. In the MP study groups, lower levels of transaminases, lactate dehydrogenase (LDH), and thiobarbituric acid reactive substances were measured compared to SCS. In parallel, mitochondrial oxygen consumption and adenosine triphosphate (ATP) production were significantly higher in the MP groups. Biomarkers of biliary function, including bile production, biliary bicarbonate concentration, and pH, were significantly higher in the MP groups, whereas biomarkers of biliary epithelial injury (biliary gamma-glutamyltransferase [GGT] and LDH), were significantly lower in MP preserved livers. Histological analysis revealed less injury of large bile duct epithelium in the MP groups compared to SCS. In conclusion, compared to SCS, end-ischemic oxygenated MP of DCD livers provides better preservation of biliary epithelial function and morphology, independent of the temperature at which MP is performed. End-ischemic oxygenated MP could reduce biliary injury after DCD liver transplantation.<br /> (© 2015 American Association for the Study of Liver Diseases.)
- Subjects :
- Adenosine Triphosphate metabolism
Animals
Bile Duct Diseases etiology
Bile Duct Diseases metabolism
Bile Duct Diseases pathology
Bile Ducts pathology
Biomarkers metabolism
Energy Metabolism
Hepatectomy
Liver Transplantation adverse effects
Male
Mitochondria metabolism
Oxygen metabolism
Oxygen Consumption
Perfusion adverse effects
Rats, Inbred Lew
Reperfusion Injury etiology
Reperfusion Injury metabolism
Reperfusion Injury pathology
Temperature
Time Factors
Bile Duct Diseases prevention & control
Bile Ducts metabolism
Cold Ischemia adverse effects
Liver Transplantation methods
Oxygen administration & dosage
Perfusion methods
Reperfusion Injury prevention & control
Warm Ischemia adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1527-6473
- Volume :
- 21
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
- Publication Type :
- Academic Journal
- Accession number :
- 26097213
- Full Text :
- https://doi.org/10.1002/lt.24200