Affinity Purification of Protein Complexes Using TAP Tags.

Authors :: Gerace E
Moazed D
Source :: Methods in enzymology [Methods Enzymol] 2015; Vol. 559, pp. 37-52. Date of Electronic Publication: 2015 Mar 29.
Publication Year :: 2015
Abstract: This protocol is used for the isolation and analysis of protein complexes using the tandem affinity purification (TAP) tag system. The protocol describes the purification of a protein fused to a TAP tag comprised of two protein A domains and the calmodulin binding peptide separated by a TEV cleavage site. This is a powerful technique for rapid purification of protein complexes and the analysis of their stoichiometric composition, posttranslational modifications, structure, and functional activities.<br /> (© 2015 Elsevier Inc. All rights reserved.)