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Affinity Purification of Protein Complexes Using TAP Tags.

Authors :
Gerace E
Moazed D
Source :
Methods in enzymology [Methods Enzymol] 2015; Vol. 559, pp. 37-52. Date of Electronic Publication: 2015 Mar 29.
Publication Year :
2015

Abstract

This protocol is used for the isolation and analysis of protein complexes using the tandem affinity purification (TAP) tag system. The protocol describes the purification of a protein fused to a TAP tag comprised of two protein A domains and the calmodulin binding peptide separated by a TEV cleavage site. This is a powerful technique for rapid purification of protein complexes and the analysis of their stoichiometric composition, posttranslational modifications, structure, and functional activities.<br /> (© 2015 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1557-7988
Volume :
559
Database :
MEDLINE
Journal :
Methods in enzymology
Publication Type :
Academic Journal
Accession number :
26096502
Full Text :
https://doi.org/10.1016/bs.mie.2014.11.007