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[Effects of conditioned media for rat bone marrow-derived mesenchymal stem cells on palmitic acid-induced insulin resistance in HepG2 cells].
- Source :
-
Zhonghua nei ke za zhi [Zhonghua Nei Ke Za Zhi] 2015 May; Vol. 54 (5), pp. 439-44. - Publication Year :
- 2015
-
Abstract
- Objective: To study the effect of conditioned media for rat bone marrow mesenchymal stem cells (BMSCs-CM) on palmitic acid (PA)-induced insulin resistance (IR) in HepG2 cells and its underlying molecular mechanisms.<br />Methods: HepG2 cells were treated with or without BMSCs-CM and L-DMEM in the presence or absence of PA.Glucose utilization in HepG2 cells were detected with PAS, glucose and glycogen measurements. Western blotting was used to assess the expression of phospho-insulin receptor substrate (p-IRS), phosphatidylinositol 3-kinase (PI3K) and p-AKT.<br />Results: (1) Incubation of HepG2 cells with 0.25 mmol/L PA for 24 hours significantly increased the glucose concentration and decreased the glycogen content (P<0.05) in the media. (2) Treatment with BMSCs-CM significantly ameliorated the glucose and glycogen alteration in cells pretreated with PA (P<0.05), however, no obvious effect of BMSCs-CM on the cell glucose and glycogen production. (3) BMSCs-CM treatment also increased protein expression of p-IRS, PI3K and p-AKT in PA incubated HapG2 cells (P<0.05). The effect of BMSCs-CM on PI3K and p-AKT expression could be mimicked upon addition of 740Y-P, a PI3K agonist, but abolished by LY294002, a PI3K specific inhibitor.<br />Conclusions: BMSCs-CM could improve the insulin sensitivity in HepG2 cells pretreated with PA through upregulation of insulin signaling component expression.
- Subjects :
- Animals
Bone Marrow
Chromones
Culture Media, Conditioned
Humans
Insulin
Insulin Resistance
Mesenchymal Stem Cells cytology
Morpholines
Palmitic Acid
Phosphatidylinositol 3-Kinases
Rats
Receptor, Insulin
Signal Transduction
Up-Regulation
Bone Marrow Cells cytology
Glucose metabolism
Hep G2 Cells drug effects
Mesenchymal Stem Cell Transplantation
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0578-1426
- Volume :
- 54
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Zhonghua nei ke za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 26080825