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Putative Serine Protease Effectors of Clavibacter michiganensis Induce a Hypersensitive Response in the Apoplast of Nicotiana Species.
- Source :
-
Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2015 Nov; Vol. 28 (11), pp. 1216-26. Date of Electronic Publication: 2015 Oct 02. - Publication Year :
- 2015
-
Abstract
- Clavibacter michiganensis subspp. michiganensis and sepedonicus cause diseases on solanaceous crops. The genomes of both subspecies encode members of the pat-1 family of putative serine proteases known to function in virulence on host plants and induction of hypersensitive responses (HR) on nonhosts. One gene of this family in C. michiganensis subsp. sepedonicus, chp-7, is required for triggering HR in Nicotiana tabacum. Here, further investigation revealed that mutation of the putative catalytic serine residue at position 232 to threonine abolished the HR induction activity of Chp-7, suggesting that enzymatic activity is required. Purified Chp-7 triggered an HR in N. tabacum leaves in the absence of the pathogen, indicating Chp-7 itself is the HR elicitor from C. michiganensis subsp. sepedonicus. Ectopic expression of chp-7 constructs in N. tabacum leaves revealed that Chp-7 targeted to the apoplast triggered an HR while cytoplasmic Chp-7 did not, indicating that Chp-7 induces the HR in the apoplast of N. tabacum leaves. Chp-7 also induced HR in N. sylvestris, a progenitor of N. tabacum, but not in other Nicotiana species tested. ChpG, a related protein from C. michiganensis subsp. michiganensis, also triggered HR in N. tabacum and N. sylvestris. Unlike Chp-7, ChpG triggered HR in N. clevelandii and N. glutinosa.
- Subjects :
- Actinobacteria genetics
Actinobacteria pathogenicity
Amino Acid Sequence
Cell Wall genetics
Cell Wall immunology
Host-Pathogen Interactions immunology
Immunoblotting
Molecular Sequence Data
Plant Diseases genetics
Plant Diseases microbiology
Plant Leaves genetics
Plant Leaves immunology
Plant Leaves microbiology
Point Mutation
Proteins genetics
Proteins metabolism
Sequence Homology, Amino Acid
Serine Proteases genetics
Serine Proteases metabolism
Species Specificity
Nicotiana classification
Nicotiana genetics
Nicotiana microbiology
Virulence genetics
Virulence immunology
Actinobacteria immunology
Plant Diseases immunology
Proteins immunology
Serine Proteases immunology
Nicotiana immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0894-0282
- Volume :
- 28
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Molecular plant-microbe interactions : MPMI
- Publication Type :
- Academic Journal
- Accession number :
- 26075829
- Full Text :
- https://doi.org/10.1094/MPMI-02-15-0036-R