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Calcium signalling in human neutrophil cell lines is not affected by low-frequency electromagnetic fields.
- Source :
-
Bioelectromagnetics [Bioelectromagnetics] 2015 Sep; Vol. 36 (6), pp. 430-43. Date of Electronic Publication: 2015 Jun 13. - Publication Year :
- 2015
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Abstract
- We are increasingly exposed to low-frequency electromagnetic fields (LF EMFs) by electrical devices and power lines, but if and how these fields interact with living cells remains a matter of debate. This study aimed to investigate the potential effect of LF EMF exposure on calcium signalling in neutrophils. In neutrophilic granulocytes, activation of G-protein coupled receptors leads to efflux of calcium from calcium stores and influx of extracellular calcium via specialised calcium channels. The cytoplasmic rise of calcium induces cytoskeleton rearrangements, modified gene expression patterns, and cell migration. If LF EMF modulates intracellular calcium signalling, this will influence cellular behaviour and may eventually lead to health problems. We found that calcium mobilisation upon chemotactic stimulation was not altered after a short 30 min or long-term LF EMF exposure in human neutrophil-like cell lines HL-60 or PLB-985. Neither of the two investigated wave forms (Immunent and 50 Hz sine wave) at three magnetic flux densities (5 μT, 300 μT, and 500 μT) altered calcium signalling in vitro. Gene-expression patterns of calcium-signalling related genes also did not show any significant changes after exposure. Furthermore, analysis of the phenotypical appearance of microvilli by scanning electron microscopy revealed no alterations induced by LF EMF exposure. The findings above indicate that exposure to 50 Hz sinusoidal or Immunent LF EMF will not affect calcium signalling in neutrophils in vitro.<br /> (© 2015 Wiley Periodicals, Inc.)
- Subjects :
- Biological Transport radiation effects
Calcium metabolism
Calcium Channels genetics
Cell Line
Gene Expression Regulation radiation effects
Humans
Kinetics
Microvilli metabolism
Microvilli radiation effects
Microvilli ultrastructure
Neutrophils metabolism
Neutrophils ultrastructure
Phenotype
RNA, Messenger genetics
RNA, Messenger metabolism
Receptors, Calcium-Sensing genetics
Time Factors
Calcium Signaling radiation effects
Electromagnetic Fields adverse effects
Neutrophils cytology
Neutrophils radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 1521-186X
- Volume :
- 36
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Bioelectromagnetics
- Publication Type :
- Academic Journal
- Accession number :
- 26073662
- Full Text :
- https://doi.org/10.1002/bem.21924