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Hepatic biotransformation and antioxidant enzyme activities in Mediterranean fish from different habitat depths.
- Source :
-
The Science of the total environment [Sci Total Environ] 2015 Nov 01; Vol. 532, pp. 176-83. Date of Electronic Publication: 2015 Jun 09. - Publication Year :
- 2015
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Abstract
- Marine fish are threatened by anthropogenic chemical discharges. However, knowledge on adverse effects on deep-sea fish or their detoxification capabilities is limited. Herein, we compared the basal activities of selected hepatic detoxification enzymes in several species (Solea solea, Dicentrarchus labrax, Trachyrhynchus scabrus, Mora moro, Cataetix laticeps and Alepocehalus rostratus) collected from the coast, middle and lower slopes of the Blanes Canyon region (Catalan continental margin, NW Mediterranean Sea). The xenobiotic-detoxifying enzymes analysed were the phase-I carboxylesterases (CbEs), and the phase-II conjugation activities uridine diphosphate glucuronyltransferase (UDPGT) and glutathione S-transferase (GST). Moreover, some antioxidant enzyme activities, i.e., catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GR), were also included in this comparative study. Because CbE activity is represented by multiple isoforms, the substrates α-naphthyl acetate (αNA) and ρ-nitrophenyl acetate (ρNPA) were used in the enzyme assays, and in vitro inhibition kinetics with dichlorvos were performed to compare interspecific CbE sensitivity. Activity of xenobiotic detoxification enzymes varied among the species, following a trend with habitat depth and body size. Thus, UDPGT and some antioxidant enzyme activities decreased in fish inhabiting lower slopes of deep-sea, whereas UDPGT and αNA-CbE activities were negatively related to fish size. A trend between CbE activities and the IC50 values for dichlorvos suggested S. solea and M. moro as potentially more sensitive to anticholinesterasic pesticides, and T. scabrus as the most resistant one. A principal component analysis considering all enzyme activities clearly identified the species but this grouping was not related to habitat depth or phylogeny. Although these results can be taken as baseline levels of the main xenobiotic detoxification enzymes in Mediterranean fish, further research is needed to evaluate their response to environmental contaminant exposure.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Catalase metabolism
Flatfishes metabolism
Glutathione Peroxidase metabolism
Glutathione Reductase metabolism
Glutathione Transferase metabolism
Naphthols metabolism
Water Pollutants, Chemical metabolism
Xenobiotics metabolism
Biotransformation
Flatfishes physiology
Liver enzymology
Water Pollutants, Chemical toxicity
Xenobiotics toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1026
- Volume :
- 532
- Database :
- MEDLINE
- Journal :
- The Science of the total environment
- Publication Type :
- Academic Journal
- Accession number :
- 26070027
- Full Text :
- https://doi.org/10.1016/j.scitotenv.2015.06.001