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Production of prostaglandin E2 induced by cigarette smoke modulates tissue factor expression and activity in endothelial cells.
- Source :
-
FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2015 Sep; Vol. 29 (9), pp. 4001-10. Date of Electronic Publication: 2015 Jun 11. - Publication Year :
- 2015
-
Abstract
- Cigarette smoke (CS) increases the incidence of atherothrombosis, the release of prostaglandin (PG) E2, and the amount of tissue factor (TF). The link between PGE2 and TF, and the impact of this interaction on CS-induced thrombosis, is unknown. Plasma from active smokers showed higher concentration of PGE2, TF total antigen, and microparticle-associated TF (MP-TF) activity compared with never smokers. Similar results were obtained in mice and in mouse cardiac endothelial cells (MCECs) after treatment with aqueous CS extracts (CSEs) plus IL-1β [CSE (6.4 puffs/L)/IL-1β (2 μg/L)]. A significant correlation between PGE2 and TF total antigen or MP-TF activity were observed in both human and mouse plasma or tissue. Inhibition of PGE synthase reduced TF in vivo and in vitro and prevented the arterial thrombosis induced by CSE/IL-1β. Only PG E receptor 1 (EP1) receptor antagonists (SC51089:IC50 ∼ 1 μM, AH6809:IC50 ∼ 7.5 μM) restored the normal TF and sirtuin 1 (SIRT1) levels in MCECs before PGE2 (EC50 ∼ 2.5 mM) or CSE/IL-1β exposure. Similarly, SIRT1 activators (CAY10591: IC50 ∼ 10 μM, resveratrol: IC50 ∼ 5 μM) or prostacyclin analogs (IC50 ∼ 5 μM) prevented SIRT1 inhibition and reduced TF induced by CSE/IL-1β or by PGE2. In conclusion, PGE2 increases both TF expression and activity through the regulation of the EP1/SIRT1 pathway. These findings suggest that EP1 may represent a possible target to prevent prothrombotic states.<br /> (© FASEB.)
- Subjects :
- Adult
Aged
Aged, 80 and over
Animals
Endothelial Cells pathology
Female
Humans
Hydrazines pharmacology
Interleukin-1beta blood
Male
Mice
Middle Aged
Myocardium metabolism
Myocardium pathology
Oxazepines pharmacology
Sirtuin 1 antagonists & inhibitors
Sirtuin 1 metabolism
Smoking adverse effects
Smoking pathology
Thrombosis etiology
Xanthones pharmacology
Dinoprostone blood
Endothelial Cells metabolism
Gene Expression Regulation
Smoking blood
Thromboplastin biosynthesis
Thrombosis blood
Subjects
Details
- Language :
- English
- ISSN :
- 1530-6860
- Volume :
- 29
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology
- Publication Type :
- Academic Journal
- Accession number :
- 26065856
- Full Text :
- https://doi.org/10.1096/fj.14-268383