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Cyclosporine A Downregulates MMP-3 and MMP-13 Expression in Cultured Pterygium Fibroblasts.
- Source :
-
Cornea [Cornea] 2015 Sep; Vol. 34 (9), pp. 1137-43. - Publication Year :
- 2015
-
Abstract
- Purpose: To investigate the regulation of matrix metalloproteinase (MMP)-3 and MMP-13 expression over time and in the presence of cyclosporine A (CsA) in primary cultured human pterygium fibroblasts. We also examined the effects of CsA on cultured human pterygium fibroblasts.<br />Methods: Primary cultured human pterygium fibroblasts subjected to scratch assays were exposed to 1 and 100 µg/mL of CsA for 3 or 10 minutes. Cells were washed with Dulbecco phosphate-buffered saline, and then incubated with serum-depleted Dulbecco modified Eagle medium/F-12 medium for 48 hours. Expression levels of MMP-3 and MMP-13 proteins and the corresponding mRNA transcripts were determined by western blotting and reverse transcription polymerase chain reaction assays, respectively.<br />Results: Migration of cultured pterygium fibroblast cells was suppressed by pretreatment with CsA compared with controls in a time-dependent and dose-dependent manner (3 minutes, 50.6% ± 1.1 in 1 µg/mL, 60.0% ± 1.2 in 100 µg/mL; 10 minutes, 59.8% ± 5.7 in 1 µg/mL, 60.5 ± 2.4 in 100 µg/mL, respectively, P < 0.01). Pretreatment with CsA also reduced the mRNA (P < 0.05) and protein expression levels (P < 0.05).<br />Conclusions: CsA was actively involved in the migration of pterygium fibroblasts. Cell migration is inhibited in response to CsA through the inhibition of MMP-3 and MMP-13 expression. These findings reveal the therapeutic potential of CsA on pterygium progression. Further studies will be necessary to elucidate the precise intracellular signal mechanism responsible for CsA-induced downregulation of MMPs in pterygium fibroblasts.
- Subjects :
- Blotting, Western
Cell Movement physiology
Cells, Cultured
Dose-Response Relationship, Drug
Female
Fibroblasts metabolism
Fibroblasts pathology
Humans
Male
Matrix Metalloproteinase 13 metabolism
Matrix Metalloproteinase 3 metabolism
Pterygium metabolism
Pterygium pathology
RNA, Messenger genetics
Reverse Transcriptase Polymerase Chain Reaction
Cyclosporine pharmacology
Fibroblasts drug effects
Gene Expression Regulation physiology
Immunosuppressive Agents pharmacology
Matrix Metalloproteinase 13 genetics
Matrix Metalloproteinase 3 genetics
Pterygium drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1536-4798
- Volume :
- 34
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Cornea
- Publication Type :
- Academic Journal
- Accession number :
- 26057330
- Full Text :
- https://doi.org/10.1097/ICO.0000000000000477