Back to Search
Start Over
Junction-mediating and regulatory protein (JMY) is essential for early porcine embryonic development.
- Source :
-
The Journal of reproduction and development [J Reprod Dev] 2015; Vol. 61 (5), pp. 361-7. Date of Electronic Publication: 2015 Jun 06. - Publication Year :
- 2015
-
Abstract
- Junction-mediating and regulatory protein (JMY) is a regulator of both transcription and actin filament assembly. JMY is a critical nucleation-promoting factor (NPF); however, its role in the development of mammalian embryos is poorly understood. In the current study, we investigated the functional roles of the NPF JMY in porcine embryos. Porcine embryos expressed JMY mRNA and protein, and JMY protein moved from the cytoplasm to the nucleus at later embryonic developmental stages. Knockdown of JMY by RNA interference markedly decreased the rate of blastocyst development, validating its role in the development of porcine embryos. Furthermore, injection of JMY dsRNA also impaired actin and Arp2 expression, and co-injection of actin and Arp2 mRNA partially rescued blastocyst development. Taken together, our results show that the NPF JMY is involved in the development of porcine embryos by regulating the NPF-Arp2-actin pathway.
- Subjects :
- Actin-Related Protein 2 genetics
Actin-Related Protein 2 metabolism
Actins genetics
Actins metabolism
Active Transport, Cell Nucleus
Animals
Blastocyst cytology
Female
Gene Knockdown Techniques veterinary
Humans
In Vitro Oocyte Maturation Techniques veterinary
Microinjections veterinary
Morula cytology
Nuclear Proteins antagonists & inhibitors
Nuclear Proteins genetics
Parthenogenesis
RNA Interference
RNA, Double-Stranded metabolism
RNA, Messenger metabolism
Sus scrofa embryology
Trans-Activators antagonists & inhibitors
Trans-Activators genetics
Blastocyst metabolism
Ectogenesis
Gene Expression Regulation, Developmental
Morula metabolism
Nuclear Proteins metabolism
Sus scrofa metabolism
Trans-Activators metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1348-4400
- Volume :
- 61
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of reproduction and development
- Publication Type :
- Academic Journal
- Accession number :
- 26052154
- Full Text :
- https://doi.org/10.1262/jrd.2015-021