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Biocontrol Activity of Bacillus subtilis Isolated from Agaricus bisporus Mushroom Compost Against Pathogenic Fungi.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2015 Jul 08; Vol. 63 (26), pp. 6009-18. Date of Electronic Publication: 2015 Jun 22. - Publication Year :
- 2015
-
Abstract
- Bacillus subtilis strain B154, isolated from Agaricus bisporus mushroom compost infected by red bread mold, exhibited antagonistic activities against Neurospora sitophila. Antifungal activity against phytopathogenic fungi was also observed. The maximum antifungal activity was reached during the stationary phase. This antifungal activity was stable over a wide pH and temperature range and was not affected by proteases. Assay of antifungal activity in vitro indicated that a purified antifungal substance could strongly inhibit mycelia growth and spore germination of N. sitophila. In addition, treatment with strain B154 in A. bisporus mushroom compost infected with N. sitophila significantly increased the yield of bisporus mushrooms. Ultraviolet scan spectroscopy, tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis, matrix-associated laser desorption ionization time-of-flight mass spectrometry, and electrospray ionization tandem mass spectrometry analyses revealed a molecular weight consistent with 1498.7633 Da. The antifungal compound might belong to a new type of lipopeptide fengycin.
- Subjects :
- Antifungal Agents chemistry
Antifungal Agents metabolism
Bacillus subtilis genetics
Bacillus subtilis isolation & purification
Bacillus subtilis metabolism
Mass Spectrometry
Molecular Weight
Neurospora growth & development
Soil chemistry
Vegetables growth & development
Agaricus growth & development
Antifungal Agents pharmacology
Bacillus subtilis chemistry
Neurospora drug effects
Soil Microbiology
Vegetables microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 63
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 26050784
- Full Text :
- https://doi.org/10.1021/acs.jafc.5b02218