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Rbbp7 Is Required for Uterine Stromal Decidualization in Mice.
- Source :
-
Biology of reproduction [Biol Reprod] 2015 Jul; Vol. 93 (1), pp. 13. Date of Electronic Publication: 2015 Jun 03. - Publication Year :
- 2015
-
Abstract
- Uterine stromal cells undergo extensive proliferation and differentiation during postimplantation development, a process known as decidualization. While a range of signaling molecules have been demonstrated to play essential roles in this event, its potential epigenetic regulatory mechanisms remain largely unknown. Retinoblastoma binding protein 7 (Rbbp7) is a protein reported as a core component of many histone modification and chromatin remodeling complexes. In the present study, our in situ hybridization and immunochemistry analysis first reveals a spatiotemporal expression of Rbbp7 in the uterus during the peri-implantation period. Observations of remarkable induction of Rbbp7 expression in uterine stromal cells in response to progesterone-nuclear receptor PR signaling point to its potential physiological significance during postimplantation uterine development. Employing a stealth RNA knockdown approach, combined with primary murine uterine stromal cell culture and an in vitro-induced decidualization model, we further demonstrate that Rbbp7 silencing compromises stromal cell decidualization via attenuating histone H4 acetylation and cyclin D3 expression. The results collectively suggest that Rbbp7 is a potentially functional player regulating normal histone acetylation modification and cyclin D3 expression in stromal cells during postimplantation decidual development.<br /> (© 2015 by the Society for the Study of Reproduction, Inc.)
Details
- Language :
- English
- ISSN :
- 1529-7268
- Volume :
- 93
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biology of reproduction
- Publication Type :
- Academic Journal
- Accession number :
- 26040671
- Full Text :
- https://doi.org/10.1095/biolreprod.115.129015