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Host tissue and glycan binding specificities of avian viral attachment proteins using novel avian tissue microarrays.
- Source :
-
PloS one [PLoS One] 2015 Jun 02; Vol. 10 (6), pp. e0128893. Date of Electronic Publication: 2015 Jun 02 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- The initial interaction between viral attachment proteins and the host cell is a critical determinant for the susceptibility of a host for a particular virus. To increase our understanding of avian pathogens and the susceptibility of poultry species, we developed novel avian tissue microarrays (TMAs). Tissue binding profiles of avian viral attachment proteins were studied by performing histochemistry on multi-species TMA, comprising of selected tissues from ten avian species, and single-species TMAs, grouping organ systems of each species together. The attachment pattern of the hemagglutinin protein was in line with the reported tropism of influenza virus H5N1, confirming the validity of TMAs in profiling the initial virus-host interaction. The previously believed chicken-specific coronavirus (CoV) M41 spike (S1) protein displayed a broad attachment pattern to respiratory tissues of various avian species, albeit with lower affinity than hemagglutinin, suggesting that other avian species might be susceptible for chicken CoV. When comparing tissue-specific binding patterns of various avian coronaviral S1 proteins on the single-species TMAs, chicken and partridge CoV S1 had predominant affinity for the trachea, while pigeon CoV S1 showed marked preference for lung of their respective hosts. Binding of all coronaviral S1 proteins was dependent on sialic acids; however, while chicken CoV S1 preferred sialic acids type I lactosamine (Gal(1-3)GlcNAc) over type II (Gal(1-4)GlcNAc), the fine glycan specificities of pigeon and partridge CoVs were different, as chicken CoV S1-specific sialylglycopolymers could not block their binding to tissues. Taken together, TMAs provide a novel platform in the field of infectious diseases to allow identification of binding specificities of viral attachment proteins and are helpful to gain insight into the susceptibility of host and organ for avian pathogens.
- Subjects :
- Animals
Chickens virology
Columbidae virology
Coronavirus metabolism
Disease Susceptibility
Host Specificity
Respiratory System virology
Tissue Array Analysis
Coronavirus pathogenicity
Galliformes virology
Hemagglutinin Glycoproteins, Influenza Virus metabolism
Host-Pathogen Interactions
Spike Glycoprotein, Coronavirus metabolism
Virus Attachment
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 26035584
- Full Text :
- https://doi.org/10.1371/journal.pone.0128893