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Phage T4-induced DNA breaks activate a tRNA repair-defying anticodon nuclease.
- Source :
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Molecular microbiology [Mol Microbiol] 2015 Sep; Vol. 97 (5), pp. 898-910. Date of Electronic Publication: 2015 Jun 26. - Publication Year :
- 2015
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Abstract
- The natural role of the conserved bacterial anticodon nuclease (ACNase) RloC is not known, but traits that set it apart from the homologous phage T4-excluding ACNase PrrC could provide relevant clues. PrrC is silenced by a genetically linked DNA restriction-modification (RM) protein and turned on by a phage-encoded DNA restriction inhibitor. In contrast, RloC is rarely linked to an RM protein, and its ACNase is regulated by an internal switch responsive to double-stranded DNA breaks. Moreover, PrrC nicks the tRNA substrate, whereas RloC excises the wobble nucleotide. These distinctions suggested that (i) T4 and related phage that degrade their host DNA will activate RloC and (ii) the tRNA species consequently disrupted will not be restored by phage tRNA repair enzymes that counteract PrrC. Consistent with these predictions we show that Acinetobacter baylyi RloC expressed in Escherichia coli is activated by wild-type phage T4 but not by a mutant impaired in host DNA degradation. Moreover, host and T4 tRNA species disrupted by the activated ACNase were not restored by T4's tRNA repair system. Nonetheless, T4's plating efficiency was inefficiently impaired by AbaRloC, presumably due to a decoy function of the phage encoded tRNA target, the absence of which exacerbated the restriction.<br /> (© 2015 John Wiley & Sons Ltd.)
- Subjects :
- Acinetobacter genetics
Acinetobacter virology
Amino Acid Sequence
Bacteriophage T4 growth & development
Bacteriophage T4 metabolism
Bacteriophage T4 pathogenicity
Enzyme Activation
Escherichia coli genetics
Escherichia coli virology
Escherichia coli Proteins genetics
RNA, Transfer genetics
Ribonucleases genetics
Bacteriophage T4 genetics
DNA Breaks
RNA, Transfer metabolism
Ribonucleases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2958
- Volume :
- 97
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Molecular microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 26031711
- Full Text :
- https://doi.org/10.1111/mmi.13074