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Seroprevalence of equine granulocytic anaplasmosis and lyme borreliosis in Canada as determined by a point-of-care enzyme-linked immunosorbent assay (ELISA).
- Source :
-
The Canadian veterinary journal = La revue veterinaire canadienne [Can Vet J] 2015 Jun; Vol. 56 (6), pp. 575-80. - Publication Year :
- 2015
-
Abstract
- Equine granulocytic anaplasmosis (EGA) and Lyme borreliosis (LB) are an emerging concern in Canada. We estimated the seroprevalence of EGA and equine LB by testing 376 convenience serum samples from 3 provinces using a point-of-care SNAP(®) 4Dx(®) ELISA (IDEXX Laboratories, Westbrook, Maine, USA), and investigated the agreement between the point-of-care ELISA and laboratory-based serologic tests. The estimated seroprevalence for EGA was 0.53% overall (0.49% in Saskatchewan, 0.71% in Manitoba), while the estimated seroprevalence for LB was 1.6% overall (0.49% in Saskatchewan, 2.86% in Manitoba). There was limited agreement between the point-of-care ELISA and an indirect fluorescent antibody test for EGA (kappa 0.1, PABAK 0.47) and an ELISA/Western blot combination for LB (kappa 0.23, PABAK 0.71). While the SNAP(®) 4Dx(®) ELISA yielded expected seroprevalence estimates, further evaluation of serologic tests for the purposes of disease exposure recognition may be needed.
- Subjects :
- Anaplasmosis blood
Animals
Canada epidemiology
Enzyme-Linked Immunosorbent Assay methods
Horse Diseases epidemiology
Horses
Lyme Disease blood
Lyme Disease epidemiology
Sensitivity and Specificity
Seroepidemiologic Studies
Anaplasmosis epidemiology
Enzyme-Linked Immunosorbent Assay veterinary
Horse Diseases blood
Lyme Disease veterinary
Point-of-Care Systems
Subjects
Details
- Language :
- English
- ISSN :
- 0008-5286
- Volume :
- 56
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Canadian veterinary journal = La revue veterinaire canadienne
- Publication Type :
- Academic Journal
- Accession number :
- 26028677