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Physiological characterization of the human EndoC-βH1 β-cell line.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2015 Aug 14; Vol. 464 (1), pp. 13-9. Date of Electronic Publication: 2015 May 28. - Publication Year :
- 2015
-
Abstract
- In the new human EndoC-βH1 β-cell line, a detailed analysis of the physiological characteristics was performed. This new human β-cell line expressed all target structures on the gene and protein level, which are crucial for physiological function and insulin secretion induced by glucose and other secretagogues. Glucose influx measurements revealed an excellent uptake capacity of EndoC-βH1 β-cells by the Glut1 and Glut2 glucose transporters. A high expression level of glucokinase enabled efficient glucose phosphorylation, increasing the ATP/ADP ratio along with stimulation of insulin secretion in the physiological glucose concentration range. The EC50 value of glucose for insulin secretion was 10.3 mM. Mannoheptulose, a specific glucokinase inhibitor, blocked glucose-induced insulin secretion (GSIS). The nutrient insulin secretagogues l-leucine and 2-ketoisocaproate also stimulated insulin secretion, with a potentiating effect of l-glutamine. The Kir 6.2 potassium channel blocker glibenclamide and Bay K 8644, an opener of the voltage-sensitive Ca(2+) channel significantly potentiated GSIS. Potentiation of GSIS by IBMX and forskolin went along with a strong stimulation of cAMP generation. In conclusion, the new human EndoC-βH1 β-cell line fully mirrors the analogous physiological characteristics of primary mouse, rat and human β-cells. Thus, this new human EndoC-βH1 β-cell line is very well suited for physiological β-cell studies.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Subjects :
- 1-Methyl-3-isobutylxanthine pharmacology
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester pharmacology
Adenosine Diphosphate metabolism
Adenosine Triphosphate biosynthesis
Biological Transport
Calcium Channels genetics
Calcium Channels metabolism
Cell Line
Colforsin pharmacology
Gene Expression
Glucokinase antagonists & inhibitors
Glucokinase genetics
Glucokinase metabolism
Glucose Transporter Type 1 genetics
Glucose Transporter Type 1 metabolism
Glucose Transporter Type 2 genetics
Glucose Transporter Type 2 metabolism
Glutamine metabolism
Glutamine pharmacology
Glyburide pharmacology
Humans
Insulin-Secreting Cells cytology
Keto Acids metabolism
Keto Acids pharmacology
Leucine metabolism
Leucine pharmacology
Mannoheptulose metabolism
Mannoheptulose pharmacology
Phosphorylation
Potassium Channels, Inwardly Rectifying antagonists & inhibitors
Potassium Channels, Inwardly Rectifying genetics
Potassium Channels, Inwardly Rectifying metabolism
Founder Effect
Glucose metabolism
Insulin metabolism
Insulin-Secreting Cells physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 464
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 26028562
- Full Text :
- https://doi.org/10.1016/j.bbrc.2015.05.072