Back to Search
Start Over
Apoptosis and cell proliferation in the mouse model of embryonic death induced by Tritrichomonas foetus infection.
- Source :
-
Experimental parasitology [Exp Parasitol] 2015 Sep; Vol. 156, pp. 32-6. Date of Electronic Publication: 2015 May 28. - Publication Year :
- 2015
-
Abstract
- Bovine tritrichomonosis is a sexually transmitted disease caused by the protozoon Tritrichomonas foetus and characterised by embryonic-death and abortion. During pregnancy, the processes of cell proliferation and death play a crucial role for blastocyst implantation and the subsequent maintenance of early pregnancy, and their misbalance may lead to the abortion. In this study, we aimed to investigate whether cell proliferation and death may be altered during tritrichomonosis. For this purpose, we used pregnant BALB/c mice as an alternative experimental animal model that has successfully reproduced the infection. We analysed the immunohistochemical expression of active caspase-3 and proliferating cell nuclear (PCNA) antigens in the endometrium of infected mice. We found an increase in the number of caspase-3 positive cells in infected mice that were not pregnant at the necropsy. Besides, the number of positive proliferating cells increased in the uterine luminal epithelium of infected animals killed at 5-7 days post coitum (dpc). Pregnant infected mice killed at 8-11 dpc showed higher proliferation than control animals. We suggest that the cytopathic effect induced by T. foetus in the uteri of infected mice may induce the apoptosis of the epithelial cells and, as a result, promote a compensatory proliferative response. The information described here will be helpful to further study the pathogenesis of the bovine tritrichomonosis.<br /> (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Apoptosis
Caspase 3 analysis
Cattle
Cattle Diseases mortality
Cattle Diseases parasitology
Cell Proliferation
Disease Models, Animal
Embryo Loss parasitology
Embryo Loss pathology
Female
Fetal Diseases mortality
Fetal Diseases pathology
Fetal Diseases veterinary
Immunohistochemistry veterinary
Male
Mice
Mice, Inbred BALB C
Pregnancy
Pregnancy Complications, Parasitic mortality
Pregnancy Complications, Parasitic pathology
Protozoan Infections, Animal mortality
Uterus enzymology
Uterus pathology
Cattle Diseases pathology
Embryo Loss veterinary
Pregnancy Complications, Parasitic veterinary
Protozoan Infections, Animal pathology
Tritrichomonas foetus pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2449
- Volume :
- 156
- Database :
- MEDLINE
- Journal :
- Experimental parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 26028409
- Full Text :
- https://doi.org/10.1016/j.exppara.2015.05.013