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Mass mortality in ornamental fish, Cyprinus carpio koi caused by a bacterial pathogen, Proteus hauseri.
- Source :
-
Acta tropica [Acta Trop] 2015 Sep; Vol. 149, pp. 128-34. Date of Electronic Publication: 2015 May 28. - Publication Year :
- 2015
-
Abstract
- Moribund koi carp, Cyprinus carpio koi, from a farm with 50% cumulative mortality were sampled with the aim of isolating and detecting the causative agent. Three bacterial species viz., Citrobacter freundii (NSCF-1), Klebsiella pneumoniae (NSKP-1) and Proteus hauseri [genomospecies 3 of Proteus vulgaris Bio group 3] (NSPH-1) were isolated, identified and characterized on the basis of biochemical tests and sequencing of the 16S rDNA gene using universal bacterial primers. Challenge experiments with these isolates using healthy koi carp showed that P. hauseri induced identical clinical and pathological states within 3 d of intramuscular injection. The results suggest P. hauseri (NSPH-1) was the causative agent. In phylogenetic analysis, strain NSPH-1 formed a distinct cluster with other P. hauseri reference strains with ≥99% sequence similarity. P. hauseri isolates were found sensitive to Ampicillin, Cefalexin, Ciprofloxacin and Cefixime and resistant to Gentamycin, Oxytetracycline, Chloramphenicol, and Kanamycin. The affected fish recovered from the infection after ciprofloxacin treatment.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Anti-Bacterial Agents pharmacology
Citrobacter freundii genetics
Citrobacter freundii isolation & purification
DNA, Ribosomal genetics
Enterobacteriaceae Infections
Fisheries
India
Klebsiella Infections
Klebsiella pneumoniae genetics
Klebsiella pneumoniae isolation & purification
Microbial Sensitivity Tests
Phylogeny
Proteus drug effects
Proteus genetics
Proteus isolation & purification
Carps microbiology
Disease Outbreaks
Fish Diseases mortality
Proteus Infections mortality
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6254
- Volume :
- 149
- Database :
- MEDLINE
- Journal :
- Acta tropica
- Publication Type :
- Academic Journal
- Accession number :
- 26028178
- Full Text :
- https://doi.org/10.1016/j.actatropica.2015.05.022