[Study on secondary metabolites of endophytic fungus Arthrinium sp. A092 from Uvaria microcarpa].

Objective: To study the secondary metabolites of the endophytic fungus Arthrinium sp. A092 from Uvaria microcarpa.
Methods: The compounds were isolated and purified by silica gel column chromatography, reverse silica gel column chromatography, Sephadex LH-20 column chromatography, HPLC, preparative TLC and recrystallization. Their structures were identified by extensive analysis of their spectroscopic data.
Results: Ten compounds were isolated from the fermentation broth extract of strain A092 and identified as flemingipanic acid(1), ( - )-gynuraone(2),2-(4-methoxy-phenyl)-ethanol(3),2-hexyl-methymaleic anhydride(4), {1-[(12E, 16E)]-12,16-eicosadienoyl]-2-[ (E, E)-7, 10-octadecadienoyl]-3-stearoylglycerol} (5), mycoediketoperazine (6), libertellenone C(7),4-hydroxymethyl-4, 6-octadiene-2, 3-diol (8), dimethyl phthalate (9), and di-(2-ethyl)-hexylphthalate (10).
Conclusion: Compounds 1,3 - 5 and 8 - 10 are isolated from the genus Arthrinium for the first time.