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Structural elucidation of a polysaccharide from Chrysanthemum morifolium flowers with anti-angiogenic activity.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2015 Aug; Vol. 79, pp. 674-80. Date of Electronic Publication: 2015 May 27. - Publication Year :
- 2015
-
Abstract
- The flowers of Chrysanthemum morifolium were extracted with 5% sodium hydroxide, yielding a neutral polysaccharide (JHB0S2) with a molecular weight of 16 kDa by anion-exchange chromatography on a DEAE-cellulose column and gel permeation chromatography on a Sephacryl S-200 HR column. JHB0S2 was found to contain glucose, xylose, arabinose and galactose in molar ratio of 2.9:2.3:1.0:1.2. Through the linkage analysis, partial acid hydrolysis and NMR spectra and so forth, indicated that its backbone consisted of 1,4-linked β-Glcp, nearly 50% of which was substituted at O-6 by 1,2-linked α-Xylp terminated with Galp or Xylp. Furthermore, some of 1,2-linked α-Xylp was substituted at O-4 by α-Araf-(1→5)-α-Araf-(1→6)-β-Galp. The biological test showed that JHB0S2 could inhibit the tube formation of HMEC-1 cells.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Angiogenesis Inhibitors isolation & purification
Angiogenesis Inhibitors pharmacology
Arabinose analysis
Carbohydrate Sequence
Cell Line, Transformed
Cell Proliferation drug effects
Chromatography, Gel
Chromatography, Ion Exchange
Endothelial Cells cytology
Endothelial Cells drug effects
Galactose analysis
Glucose analysis
Humans
Hydrolysis
Molecular Sequence Data
Molecular Weight
Polysaccharides isolation & purification
Polysaccharides pharmacology
Sodium Hydroxide chemistry
Xylose analysis
Angiogenesis Inhibitors chemistry
Chrysanthemum chemistry
Flowers chemistry
Neovascularization, Physiologic drug effects
Polysaccharides chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 79
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 26025076
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2015.04.026