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Downregulation of the Host Gene jigr1 by miR-92 Is Essential for Neuroblast Self-Renewal in Drosophila.
- Source :
-
PLoS genetics [PLoS Genet] 2015 May 22; Vol. 11 (5), pp. e1005264. Date of Electronic Publication: 2015 May 22 (Print Publication: 2015). - Publication Year :
- 2015
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Abstract
- Intragenic microRNAs (miRNAs), located mostly in the introns of protein-coding genes, are often co-expressed with their host mRNAs. However, their functional interaction in development is largely unknown. Here we show that in Drosophila, miR-92a and miR-92b are embedded in the intron and 3'UTR of jigr1, respectively, and co-expressed with some jigr1 isoforms. miR-92a and miR-92b are highly expressed in neuroblasts of larval brain where Jigr1 expression is low. Genetic deletion of both miR-92a and miR-92b demonstrates an essential cell-autonomous role for these miRNAs in maintaining neuroblast self-renewal through inhibiting premature differentiation. We also show that miR-92a and miR-92b directly target jigr1 in vivo and that some phenotypes due to the absence of these miRNAs are partially rescued by reducing the level of jigr1. These results reveal a novel function of the miR-92 family in Drosophila neuroblasts and provide another example that local negative feedback regulation of host genes by intragenic miRNAs is essential for animal development.
- Subjects :
- 3' Untranslated Regions
Animals
Brain embryology
Brain metabolism
DNA-Binding Proteins genetics
Drosophila Proteins genetics
Gene Expression Regulation, Developmental
Larva genetics
Larva metabolism
Male
MicroRNAs genetics
Neural Stem Cells metabolism
Neuroglia cytology
Neuroglia metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Sequence Alignment
DNA-Binding Proteins metabolism
Down-Regulation
Drosophila genetics
Drosophila Proteins metabolism
MicroRNAs metabolism
Neural Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7404
- Volume :
- 11
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- PLoS genetics
- Publication Type :
- Academic Journal
- Accession number :
- 26000445
- Full Text :
- https://doi.org/10.1371/journal.pgen.1005264