An aptasensor for selective, sensitive and fast detection of lead(II) based on polyethyleneimine and gold nanoparticles.

Authors :: Taghdisi SM
Danesh NM
Lavaee P
Ramezani M
Abnous K
Source :: Environmental toxicology and pharmacology [Environ Toxicol Pharmacol] 2015 May; Vol. 39 (3), pp. 1206-11. Date of Electronic Publication: 2015 Apr 27.
Publication Year :: 2015
Abstract: Lead (Pb), as a major environmental contaminant, could be harmful to humans when inhaled or ingested. In this study, we developed a sensitive, selective and fast colorimetric aptasensor for Pb(+2) based on polyethylenimine (PEI) and gold nanoparticles (AuNPs). In the absence of Pb(+2), aptamer binds to PEI. So the well-dispersed AuNPs remain stable with a wine-red color. Upon the addition of Pb(+2), a conformational change happens and a G-quadruplex aptamer/Pb(+2) complex is formed, leading to the aggregation of AuNPs and a color change to blue. This sensor showed a high selectivity toward Pb(+2) with a limit of detection (LOD) as low as 702pM. Moreover, our fabricated sensor was successfully applied for Pb(+2) detection in rat serum and tap water.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)

Subjects :: Animals
Aptamers, Nucleotide metabolism
Biosensing Techniques methods
Environmental Monitoring methods
Lead blood
Limit of Detection
Metal Nanoparticles chemistry
Models, Chemical
Rats
Water Pollutants, Chemical analysis
Aptamers, Nucleotide chemistry
Colorimetry methods
Gold chemistry
Lead analysis
Polyethyleneimine metabolism

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