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Urinary leukotriene and Bcl I polymorphism of glucocorticoid receptor gene in preschoolers with recurrent wheezing and high risk of asthma.
- Source :
-
Allergologia et immunopathologia [Allergol Immunopathol (Madr)] 2016 Jan-Feb; Vol. 44 (1), pp. 59-65. Date of Electronic Publication: 2015 May 13. - Publication Year :
- 2016
-
Abstract
- Background: Urinary leukotriene (LTE4) is an important marker of airway inflammation presence. A relationship between single nucleotide polymorphism in the glucocorticoid receptor (GCR) gene promoter (Bcl I polymorphism), development of asthma and sensitivity to glucocorticoids has been hypothesised.<br />Objective: To explore the possible association between the Bcl I polymorphism and baseline levels of urinary LTE4 in preschoolers with recurrent wheezing episodes. We prospectively enrolled and classified 86 preschoolers based on the risk of developing asthma (by the Asthma Predictive Index [API]).<br />Methods: At admission standardised questionnaires for demographics and respiratory illness characteristics were completed. The Bcl I polymorphism of the GCR was determined by a PCR-RFLP assay from blood samples, and urinary leukotriene was assessed from urine samples by an enzyme immunoassay.<br />Results: We enrolled 86 preschoolers (46 with positive API and 40 with negative API). There were no statistical differences in demographic, respiratory illnesses and wheezing episodes characteristics between both groups. Also, the prevalence of Bcl I polymorphism was similar between positive vs. negative API groups (34.8% vs. 38.9% for homozygote GG, 56.5% vs. 52.8% for heterozygote GC, 8.7% vs. 8.3% for homozygote CC, respectively, p=0.94). However, urinary LTE4 (median [IQR]) was higher in preschoolers with positive than negative API (7.18 [5.57-8.96pg/ml] vs. 6.42 [3.96-8.07pg/ml], p=0.02, respectively).<br />Conclusions: In our population, wheezing preschoolers with positive API exhibit higher levels of urinary LTE4 than those with negative API; but there were no differences in Bcl I polymorphism of the GCR.<br /> (Copyright © 2015 SEICAP. Published by Elsevier Espana. All rights reserved.)
- Subjects :
- Asthma complications
Asthma drug therapy
Biomarkers, Pharmacological metabolism
Case-Control Studies
Child, Preschool
DNA Mutational Analysis
Female
Glucocorticoids therapeutic use
Humans
Leukotriene E4 blood
Male
Polymorphism, Single Nucleotide
Prospective Studies
Recurrence
Respiratory Sounds etiology
Respiratory Sounds genetics
Risk
Surveys and Questionnaires
Asthma immunology
Leukotriene E4 genetics
Promoter Regions, Genetic genetics
Receptors, Glucocorticoid genetics
Respiratory Sounds immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1578-1267
- Volume :
- 44
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Allergologia et immunopathologia
- Publication Type :
- Academic Journal
- Accession number :
- 25982579
- Full Text :
- https://doi.org/10.1016/j.aller.2015.02.003