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A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation.
- Source :
-
Stem cell reports [Stem Cell Reports] 2015 Jun 09; Vol. 4 (6), pp. 1031-45. Date of Electronic Publication: 2015 May 14. - Publication Year :
- 2015
-
Abstract
- The utility of stem cells and their progeny in adult transplantation models has been limited by poor survival and integration. We designed an injectable and bioresorbable hydrogel blend of hyaluronan and methylcellulose (HAMC) and tested it with two cell types in two animal models, thereby gaining an understanding of its general applicability for enhanced cell distribution, survival, integration, and functional repair relative to conventional cell delivery in saline. HAMC improves cell survival and integration of retinal stem cell (RSC)-derived rods in the retina. The pro-survival mechanism of HAMC is ascribed to the interaction of the CD44 receptor with HA. Transient disruption of the retinal outer limiting membrane, combined with HAMC delivery, results in significantly improved rod survival and visual function. HAMC also improves the distribution, viability, and functional repair of neural stem and progenitor cells (NSCs). The HAMC delivery system improves cell transplantation efficacy in two CNS models, suggesting broad applicability.<br /> (Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Blindness genetics
Blindness therapy
Blindness veterinary
Cell Survival
Hyaluronan Receptors genetics
Hyaluronan Receptors metabolism
Immunohistochemistry
Methylcellulose chemistry
Mice
Mice, Inbred C57BL
Mice, Knockout
Real-Time Polymerase Chain Reaction
Retina cytology
Retina metabolism
Retinal Rod Photoreceptor Cells cytology
Rhodopsin metabolism
Stem Cells metabolism
Stroke chemically induced
Stroke therapy
Stroke veterinary
Hyaluronic Acid chemistry
Hydrogel, Polyethylene Glycol Dimethacrylate chemistry
Stem Cell Transplantation
Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 2213-6711
- Volume :
- 4
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Stem cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 25981414
- Full Text :
- https://doi.org/10.1016/j.stemcr.2015.04.008