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Administration of granulocyte colony-stimulating factor with radiotherapy promotes tumor growth by stimulating vascularization in tumor-bearing mice.
- Source :
-
Oncology reports [Oncol Rep] 2015 Jul; Vol. 34 (1), pp. 147-54. Date of Electronic Publication: 2015 May 13. - Publication Year :
- 2015
-
Abstract
- Although granulocyte-colony stimulating factor (G-CSF) is commonly used to support recovery from radiation-induced side-effects, the precise effects of G-CSF on colon cancer under radiotherapy remain poorly understood. In the present study, to investigate the effects of tumor growth following radiotherapy and G-CSF administration in a murine xenograft model of colon cancer, female BALB/c mice were injected with cells of a colon carcinoma cell line (CT26) with irradiation and G-CSF, alone or in combination. Mice received 2 Gy of focal radiation daily for 5 days and intraperitoneal injection of G-CSF (100 µg/kg/day) after irradiation for 7 days. Changes in the levels of myeloperoxidase (MPO), vascular endothelial growth factor (VEGF), matrix metalloproteinase type 9 (MMP-9) and CD31 were assessed in the mouse cancer induced by injection of colon cancer cells. We observed that G-CSF increased the number of circulating neutrophils, but facilitated tumor growth. However, G-CSF treatment did not affect radiation-induced cytotoxicity and cell viability in CT26 cells in vitro. Increased levels of myeloperoxidase, a neutrophil marker and those of vascular endothelial growth factor were observed in tumors with G-CSF supplementation. In addition, we found that increased levels of CD31 and matrix metalloproteinase-9 were correlated with the enhanced tumor growth after G-CSF treatment. Therefore, these data suggest that G-CSF may contribute to tumor growth and decrease the antitumor effect of radiotherapy, possibly by promoting vascularization in cancer lesions.
- Subjects :
- Animals
Colonic Neoplasms pathology
Female
Granulocyte Colony-Stimulating Factor administration & dosage
Granulocyte Colony-Stimulating Factor metabolism
Humans
Leukocyte Count
Matrix Metalloproteinase 9 biosynthesis
Matrix Metalloproteinase 9 genetics
Mice
Neovascularization, Pathologic drug therapy
Neovascularization, Pathologic radiotherapy
Neutrophils drug effects
Neutrophils pathology
Platelet Endothelial Cell Adhesion Molecule-1 biosynthesis
Platelet Endothelial Cell Adhesion Molecule-1 genetics
Radiation-Protective Agents metabolism
Vascular Endothelial Growth Factor A biosynthesis
Xenograft Model Antitumor Assays
Colonic Neoplasms genetics
Colonic Neoplasms radiotherapy
Granulocyte Colony-Stimulating Factor genetics
Neovascularization, Pathologic genetics
Radiation-Protective Agents administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2431
- Volume :
- 34
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 25976379
- Full Text :
- https://doi.org/10.3892/or.2015.3977