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Expansion of NK cells from PBMCs using immobilized 4-1BBL and interleukin-21.
- Source :
-
International journal of oncology [Int J Oncol] 2015 Jul; Vol. 47 (1), pp. 335-42. Date of Electronic Publication: 2015 May 14. - Publication Year :
- 2015
-
Abstract
- Adoptive transfer of NK cells has been widely applied clinically for cancer immunotherapy. However, the difficulties to obtain a large number of activated NK cells impede the successful application of such therapy. In the present study, we implemented a novel method involving the use of immobilized human 4-1BBL and interleukin-21 to amplify NK cells from the peripheral blood mononuclear cells (PBMCs) of healthy donors. Following stimulation for 21 days, we achieved considerable expansion of NK cells with high purity and strong cytotoxicity. This is the first time solid phase cytokines were used to augment NK cells, and this method has the advantage of no need to introduce feeder cells, without prior purification of NK cells and it effectively stimulated and expanded NK cells. The strategy of cell proliferation and activation could lead to a safer and more effective application of NK cells clinically.
- Subjects :
- 4-1BB Ligand chemistry
4-1BB Ligand genetics
Biotinylation
Cell Proliferation
Humans
Immobilized Nucleic Acids
Immobilized Proteins biosynthesis
Interleukins chemistry
Interleukins genetics
Lymphocyte Activation
Recombinant Proteins biosynthesis
Recombinant Proteins chemistry
Recombinant Proteins genetics
4-1BB Ligand biosynthesis
Interleukins biosynthesis
Killer Cells, Natural immunology
Leukocytes, Mononuclear cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2423
- Volume :
- 47
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of oncology
- Publication Type :
- Academic Journal
- Accession number :
- 25975533
- Full Text :
- https://doi.org/10.3892/ijo.2015.3005