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Cranberry Xyloglucan Structure and Inhibition of Escherichia coli Adhesion to Epithelial Cells.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2015 Jun 17; Vol. 63 (23), pp. 5622-33. Date of Electronic Publication: 2015 Jun 09. - Publication Year :
- 2015
-
Abstract
- Cranberry juice has been recognized as a treatment for urinary tract infections on the basis of scientific reports of proanthocyanidin anti-adhesion activity against Escherichia coli as well as from folklore. Xyloglucan oligosaccharides were detected in cranberry juice and the residue remaining following commercial juice extraction that included pectinase maceration of the pulp. A novel xyloglucan was detected through tandem mass spectrometry analysis of an ion at m/z 1055 that was determined to be a branched, three hexose, four pentose oligosaccharide consistent with an arabino-xyloglucan structure. Two-dimensional nuclear magnetic resonance spectroscopy analysis provided through-bond correlations for the α-L-Araf (1→2) α-D-Xylp (1→6) β-D-Glcp sequence, proving the S-type cranberry xyloglucan structure. Cranberry xyloglucan-rich fractions inhibited the adhesion of E. coli CFT073 and UTI89 strains to T24 human bladder epithelial cells and that of E. coli O157:H7 to HT29 human colonic epithelial cells. SSGG xyloglucan oligosaccharides represent a new cranberry bioactive component with E. coli anti-adhesion activity and high affinity for type 1 fimbriae.
- Subjects :
- Cell Line
Escherichia coli physiology
Glucans chemistry
Humans
Plant Extracts chemistry
Xylans chemistry
Bacterial Adhesion drug effects
Beverages analysis
Epithelial Cells microbiology
Escherichia coli drug effects
Glucans pharmacology
Plant Extracts pharmacology
Vaccinium macrocarpon chemistry
Xylans pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 63
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 25973733
- Full Text :
- https://doi.org/10.1021/acs.jafc.5b00730