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Expression analysis of dihydroflavonol 4-reductase genes in Petunia hybrida.
- Source :
-
Genetics and molecular research : GMR [Genet Mol Res] 2015 May 12; Vol. 14 (2), pp. 5010-21. Date of Electronic Publication: 2015 May 12. - Publication Year :
- 2015
-
Abstract
- Dihydroflavonol 4-reductase (DFR) genes from Rosa chinensis (Asn type) and Calibrachoa hybrida (Asp type), driven by a CaMV 35S promoter, were integrated into the petunia (Petunia hybrida) cultivar 9702. Exogenous DFR gene expression characteristics were similar to flower-color changes, and effects on anthocyanin concentration were observed in both types of DFR gene transformants. Expression analysis showed that exogenous DFR genes were expressed in all of the tissues, but the expression levels were significantly different. However, both of them exhibited a high expression level in petals that were starting to open. The introgression of DFR genes may significantly change DFR enzyme activity. Anthocyanin ultra-performance liquid chromatography results showed that anthocyanin concentrations changed according to DFR enzyme activity. Therefore, the change in flower color was probably the result of a DFR enzyme change. Pelargonidin 3-O-glucoside was found in two different transgenic petunias, indicating that both CaDFR and RoDFR could catalyze dihydrokaempferol. Our results also suggest that transgenic petunias with DFR gene of Asp type could biosynthesize pelargonidin 3-O-glucoside.
- Subjects :
- Alcohol Oxidoreductases genetics
Anthocyanins biosynthesis
Color
Flavonoids metabolism
Flowers anatomy & histology
Flowers enzymology
Petunia anatomy & histology
Petunia enzymology
Plant Proteins genetics
Promoter Regions, Genetic
Rosa chemistry
Rosa enzymology
Solanaceae chemistry
Solanaceae enzymology
Transgenes
Alcohol Oxidoreductases biosynthesis
Flowers genetics
Gene Expression Regulation, Plant
Petunia genetics
Plant Proteins biosynthesis
Plants, Genetically Modified
Subjects
Details
- Language :
- English
- ISSN :
- 1676-5680
- Volume :
- 14
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Genetics and molecular research : GMR
- Publication Type :
- Academic Journal
- Accession number :
- 25966276
- Full Text :
- https://doi.org/10.4238/2015.May.12.4