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Identification of two metallothioneins as novel inhalative coffee allergens cof a 2 and cof a 3.
- Source :
-
PloS one [PLoS One] 2015 May 11; Vol. 10 (5), pp. e0126455. Date of Electronic Publication: 2015 May 11 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- Background: Dust of green coffee beans is known to be a relevant cause for occupational allergic disorders in coffee industry workers. Recently, we described the first coffee allergen (Cof a 1) establishing an allergenic potential of green coffee dust.<br />Objective: Our aim was to identify allergenic components of green coffee in order to enhance inhalative coffee allergy diagnosis.<br />Methods: A Coffea arabica pJuFo cDNA phage display library was created and screened for IgE binding with sera from allergic coffee workers. Two further coffee allergens were identified by sequence analysis, expressed in E. coli, and evaluated by Western blots. The prevalence of sensitization to recombinant Cof a 1, Cof a 2, and Cof a 3 and to commercially available extract was investigated by ELISA (enzyme-linked immunosorbent assay) respectively CAP (capacity test) screening in 18 sera of symptomatic coffee workers.<br />Results: In addition to the previously described chitinase Cof a 1, two Coffea arabica cysteine-rich metallothioneins of 9 and 7 kDa were identified and included in the IUIS Allergen Nomenclature as Cof a 2 and Cof a 3. Serum IgE antibodies to at least one of the recombinant allergens were found in 8 out of 18 symptomatic coffee workers (44%). Only 2 of the analysed sera (11%) had reacted previously to the commercial allergy test.<br />Conclusions: In addition to the previously described Cof a 1 we have identified two further coffee proteins to be type I coffee allergens (Cof a 2 and Cof a 3) which may have a relevant potential for the specific diagnosis and/or therapy of coffee allergy.
- Subjects :
- Adult
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Coffea genetics
DNA, Complementary
Female
Gene Expression
Humans
Hypersensitivity immunology
Immunoglobulin E blood
Immunoglobulin E immunology
Male
Metallothionein chemistry
Metallothionein genetics
Middle Aged
Molecular Sequence Data
Occupational Diseases immunology
Plant Proteins chemistry
Plant Proteins genetics
Recombinant Proteins
Sequence Alignment
Allergens immunology
Coffea adverse effects
Coffee adverse effects
Metallothionein immunology
Plant Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 25962169
- Full Text :
- https://doi.org/10.1371/journal.pone.0126455