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Oxidized low-density lipoprotein alters endothelial progenitor cell populations.
- Source :
-
Frontiers in bioscience (Landmark edition) [Front Biosci (Landmark Ed)] 2015 Jun 01; Vol. 20 (6), pp. 975-88. Date of Electronic Publication: 2015 Jun 01. - Publication Year :
- 2015
-
Abstract
- Oxidized low-density lipoprotein (ox-LDL) is critical to atherosclerosis in hyperlipidemia. Bone marrow (BM)-derived endothelial progenitor cells (EPCs) are important to preventing atherosclerosis, and significantly decreased in hyperlipidemia. This study was to demonstrate ox-LDL and hyperlipidemia could exhibit similar effect on EPC population and the role of reactive oxygen species (ROS). ROS production in BM and blood was significantly increased in male C57BL/6 mice with intravenous ox-LDL treatment, and in hyperlipidemic LDL receptor knockout mice with 4-month high-fat diet. ROS formation was effectively blocked with overexpression of antioxidant enzymes or N-acetylcysteine treatment. In hyperlipidemic and ox-LDL-treated mice, c-Kit(+)/CD31(+) cell number in BM and blood, and Sca-1(+)/Flk-1(+) cell number in blood, not in BM, were significantly decreased, which were not affected by inhibiting ROS production, while blood CD34(+)/Flk-1(+) cell number was significantly increased that was prevented with reduced ROS formation. However, blood CD34(+)/CD133(+) cell number increased in ox-LDL-treated mice, while decreased in hyperlipidemic mice. These data suggested that ox-LDL produced significant changes in BM and blood EPC populations similar (but not identical) to chronic hyperlipidemia with predominantly ROS-independent mechanism(s).
- Subjects :
- Animals
Bone Marrow metabolism
Cells, Cultured
Endothelial Progenitor Cells metabolism
Hyperlipidemias metabolism
Hyperlipidemias pathology
Lipoproteins, LDL pharmacology
Male
Mice, Inbred C57BL
Reactive Oxygen Species metabolism
Endothelial Progenitor Cells cytology
Lipoproteins, LDL metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2768-6698
- Volume :
- 20
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Frontiers in bioscience (Landmark edition)
- Publication Type :
- Academic Journal
- Accession number :
- 25961537
- Full Text :
- https://doi.org/10.2741/4351