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Isolation and characterisation of insulin-releasing compounds from Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis venom.
- Source :
-
Toxicon : official journal of the International Society on Toxinology [Toxicon] 2015 Jul; Vol. 101, pp. 48-54. Date of Electronic Publication: 2015 May 07. - Publication Year :
- 2015
-
Abstract
- Crude venom from three venomous snakes, Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis was fractionated by gel filtration chromatography, and selected fractions screened for in-vitro insulinotropic activity using clonal pancreatic BRIN-BD11 cells. Nineteen fractions stimulated insulin secretion and the structural identity of bioactive compounds responsible was probed using MALDI-ToF MS and N-terminal Edman degradation sequencing. Partial N-terminal sequences were determined and their homology to existing sequences identified using BLAST searching. The main insulinotropic peptide families identified were made up of snake venom serine proteinases, phospholipases A2 (PLA2) and disintegrins. Snake venom constituents may have therapeutic potential for diabetes, with each of the three viper venoms tested providing insulinotropic compounds from a range of different toxin families.<br /> (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Cell Line
Chromatography, Gel
Crotalid Venoms chemistry
Crotalus classification
Disintegrins metabolism
Insulin Secretion
Insulin-Secreting Cells cytology
Insulin-Secreting Cells drug effects
Phospholipases A2 metabolism
Rats
Serine Proteases metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Viper Venoms chemistry
Viperidae classification
Crotalid Venoms pharmacology
Crotalus metabolism
Insulin metabolism
Viper Venoms pharmacology
Viperidae metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3150
- Volume :
- 101
- Database :
- MEDLINE
- Journal :
- Toxicon : official journal of the International Society on Toxinology
- Publication Type :
- Academic Journal
- Accession number :
- 25959507
- Full Text :
- https://doi.org/10.1016/j.toxicon.2015.05.002