Helicobacter pylori vesicles carrying CagA localize in the vicinity of cell-cell contacts and induce histone H1 binding to ATP in epithelial cells.

Authors :: Turkina MV
Olofsson A
Magnusson KE
Arnqvist A
Vikström E
Source :: FEMS microbiology letters [FEMS Microbiol Lett] 2015 Jun; Vol. 362 (11). Date of Electronic Publication: 2015 May 07.
Publication Year :: 2015
Abstract: Helicobacter pylori produces outer membrane vesicles (OMV), delivering bacterial substances including the oncogenic cytotoxin-associated CagA protein to their surroundings. We investigated the effects of H. pylori OMV carrying CagA (OMV-CagA) on cell junctions and ATP-binding proteome of epithelial monolayers, using proteomics, mass spectrometry and imaging. OMV-CagA localized in close vicinity of ZO-1 tight junction protein and induced histone H1 binding to ATP. We suggest the expression of novel events in the interactions between H. pylori OMV and epithelia, which may have an influence on host gene transcription and lead to different outcomes of an infection and development of cancer.<br /> (© FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Subjects :: Antigens, Bacterial genetics
Bacterial Proteins genetics
Caco-2 Cells
Helicobacter pylori genetics
Host-Pathogen Interactions
Humans
Microscopy, Confocal
Tight Junctions metabolism
Tight Junctions ultrastructure
Zonula Occludens-1 Protein metabolism
Adenosine Triphosphate metabolism
Antigens, Bacterial metabolism
Bacterial Proteins metabolism
Helicobacter pylori metabolism
Histones metabolism

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