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Microarray Analysis of Long Non-coding RNA Expression Profile Associated with 5-Fluorouracil-Based Chemoradiation Resistance in Colorectal Cancer Cells.
- Source :
-
Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2015; Vol. 16 (8), pp. 3395-402. - Publication Year :
- 2015
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Abstract
- Background: Preoperative 5-fluorouracil (5-FU)-based chemoradiotherapy is a standard treatment for locally advanced colorectal cancer (CRC). However, CRC cells often develop chemoradiation resistance (CRR). Recent studies have shown that long non-coding RNA (lncRNA) plays critical roles in a myriad of biological processes and human diseases, as well as chemotherapy resistance. Since the roles of lncRNAs in 5-FU-based CRR in human CRC cells remain unknown, they were investigated in this study.<br />Materials and Methods: A 5-FU-based concurrent CRR cell model was established using human CRC cell line HCT116. Microarray expression profiling of lncRNAs and mRNAs was undertaken in parental HCT116 and 5-FU-based CRR cell lines.<br />Results: In total, 2,662 differentially expressed lncRNAs and 2,398 mRNAs were identified in 5-FU-based CRR HCT116 cells when compared with those in parental HCT116. Moreover, 6 lncRNAs and 6 mRNAs found to be differentially expressed were validated by quantitative real time PCR (qRT-PCR). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis for the differentially expressed mRNAs indicated involvement of many, such as Jak- STAT, PI3K-Akt and NF-kappa B signaling pathways. To better understand the molecular basis of 5-FU-based CRR in CRC cells, correlated expression networks were constructed based on 8 intergenic lncRNAs and their nearby coding genes.<br />Conclusions: Changes in lncRNA expression are involved in 5-FU-based CRR in CRC cells. These findings may provide novel insight for the prognosis and prediction of response to therapy in CRC patients.
- Subjects :
- Chemoradiotherapy
Colorectal Neoplasms therapy
Gene Expression Profiling
HCT116 Cells
Humans
Janus Kinases genetics
Microarray Analysis
NF-kappa B genetics
Phosphatidylinositol 3-Kinases genetics
Proto-Oncogene Proteins c-akt genetics
RNA, Long Noncoding genetics
Real-Time Polymerase Chain Reaction
STAT Transcription Factors genetics
Signal Transduction genetics
Antineoplastic Agents
Colorectal Neoplasms genetics
Drug Resistance, Neoplasm genetics
Fluorouracil
Gene Expression Regulation, Neoplastic
RNA, Long Noncoding metabolism
RNA, Messenger metabolism
Radiation Tolerance genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2476-762X
- Volume :
- 16
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Asian Pacific journal of cancer prevention : APJCP
- Publication Type :
- Academic Journal
- Accession number :
- 25921151
- Full Text :
- https://doi.org/10.7314/apjcp.2015.16.8.3395