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Cas9-mediated targeting of viral RNA in eukaryotic cells.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2015 May 12; Vol. 112 (19), pp. 6164-9. Date of Electronic Publication: 2015 Apr 27. - Publication Year :
- 2015
-
Abstract
- Clustered, regularly interspaced, short palindromic repeats-CRISPR associated (CRISPR-Cas) systems are prokaryotic RNA-directed endonuclease machineries that act as an adaptive immune system against foreign genetic elements. Using small CRISPR RNAs that provide specificity, Cas proteins recognize and degrade nucleic acids. Our previous work demonstrated that the Cas9 endonuclease from Francisella novicida (FnCas9) is capable of targeting endogenous bacterial RNA. Here, we show that FnCas9 can be directed by an engineered RNA-targeting guide RNA to target and inhibit a human +ssRNA virus, hepatitis C virus, within eukaryotic cells. This work reveals a versatile and portable RNA-targeting system that can effectively function in eukaryotic cells and be programmed as an antiviral defense.
- Subjects :
- 3' Untranslated Regions
5' Untranslated Regions
Base Sequence
CRISPR-Associated Protein 9
Cell Line, Tumor
DNA chemistry
Epitopes chemistry
Hepacivirus
Humans
Molecular Sequence Data
Plasmids metabolism
Transfection
Viral Proteins metabolism
Bacterial Proteins metabolism
CRISPR-Associated Proteins metabolism
Endonucleases metabolism
Francisella metabolism
RNA, Viral chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 112
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 25918406
- Full Text :
- https://doi.org/10.1073/pnas.1422340112